The extraction of polychlorinated biphenyls and polyaromatic hydrocarbons from serum was studied by reversed phase liquid chromatography using restricted-access reversed-phase packing materials. The restrictive dimensions of the hydrophobic pores of the superficially hydrophilic packings excluded serum proteins and facilitated retention of the smaller hydrophobic compounds included in the buffer-acetonitrile injection mixture. Only a relatively short time was required for analytes to be separated from less hydrophobic serum components and recovered with acetonitrile elution, providing samples for GC/MS quantification. The factors involved with injections of larger volumes of serum are addressed.
|Original language||English (US)|
|Number of pages||7|
|Journal||Analytical methods & instrumentation|
|State||Published - 1995|
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