Sample preparation for GC/MS analysis of hydrophobic environmental contaminants by direct serum injection into restricted-access reversed-phase HPLC columns

Nobuo Tanaka, Kazuhiro Kimata, Ken Hosoya, Takeo Araki, Elizabeth R. Barnhart, Louis R. Alexander, Patricia C. McClure, Chester Lapeza, Donald G. Patterson

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The extraction of polychlorinated biphenyls and polyaromatic hydrocarbons from serum was studied by reversed phase liquid chromatography using restricted-access reversed-phase packing materials. The restrictive dimensions of the hydrophobic pores of the superficially hydrophilic packings excluded serum proteins and facilitated retention of the smaller hydrophobic compounds included in the buffer-acetonitrile injection mixture. Only a relatively short time was required for analytes to be separated from less hydrophobic serum components and recovered with acetonitrile elution, providing samples for GC/MS quantification. The factors involved with injections of larger volumes of serum are addressed.

Original languageEnglish (US)
Pages (from-to)41-47
Number of pages7
JournalAnalytical methods & instrumentation
Volume2
Issue number1
StatePublished - Jan 1 1995

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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    Tanaka, N., Kimata, K., Hosoya, K., Araki, T., Barnhart, E. R., Alexander, L. R., McClure, P. C., Lapeza, C., & Patterson, D. G. (1995). Sample preparation for GC/MS analysis of hydrophobic environmental contaminants by direct serum injection into restricted-access reversed-phase HPLC columns. Analytical methods & instrumentation, 2(1), 41-47.