Sample preparation for GC/MS analysis of hydrophobic environmental contaminants by direct serum injection into restricted-access reversed-phase HPLC columns

Nobuo Tanaka, Kazuhiro Kimata, Ken Hosoya, Takeo Araki, Elizabeth R. Barnhart, Louis R. Alexander, Patricia C. McClure, Chester Lapeza, Donald G. Patterson

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The extraction of polychlorinated biphenyls and polyaromatic hydrocarbons from serum was studied by reversed phase liquid chromatography using restricted-access reversed-phase packing materials. The restrictive dimensions of the hydrophobic pores of the superficially hydrophilic packings excluded serum proteins and facilitated retention of the smaller hydrophobic compounds included in the buffer-acetonitrile injection mixture. Only a relatively short time was required for analytes to be separated from less hydrophobic serum components and recovered with acetonitrile elution, providing samples for GC/MS quantification. The factors involved with injections of larger volumes of serum are addressed.

Original languageEnglish (US)
Pages (from-to)41-47
Number of pages7
JournalAnalytical methods & instrumentation
Volume2
Issue number1
StatePublished - Jan 1 1995

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Acetonitrile
Impurities
Liquid chromatography
Polychlorinated biphenyls
Hydrocarbons
Proteins

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Tanaka, N., Kimata, K., Hosoya, K., Araki, T., Barnhart, E. R., Alexander, L. R., ... Patterson, D. G. (1995). Sample preparation for GC/MS analysis of hydrophobic environmental contaminants by direct serum injection into restricted-access reversed-phase HPLC columns. Analytical methods & instrumentation, 2(1), 41-47.
Tanaka, Nobuo ; Kimata, Kazuhiro ; Hosoya, Ken ; Araki, Takeo ; Barnhart, Elizabeth R. ; Alexander, Louis R. ; McClure, Patricia C. ; Lapeza, Chester ; Patterson, Donald G. / Sample preparation for GC/MS analysis of hydrophobic environmental contaminants by direct serum injection into restricted-access reversed-phase HPLC columns. In: Analytical methods & instrumentation. 1995 ; Vol. 2, No. 1. pp. 41-47.
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Tanaka, N, Kimata, K, Hosoya, K, Araki, T, Barnhart, ER, Alexander, LR, McClure, PC, Lapeza, C & Patterson, DG 1995, 'Sample preparation for GC/MS analysis of hydrophobic environmental contaminants by direct serum injection into restricted-access reversed-phase HPLC columns', Analytical methods & instrumentation, vol. 2, no. 1, pp. 41-47.

Sample preparation for GC/MS analysis of hydrophobic environmental contaminants by direct serum injection into restricted-access reversed-phase HPLC columns. / Tanaka, Nobuo; Kimata, Kazuhiro; Hosoya, Ken; Araki, Takeo; Barnhart, Elizabeth R.; Alexander, Louis R.; McClure, Patricia C.; Lapeza, Chester; Patterson, Donald G.

In: Analytical methods & instrumentation, Vol. 2, No. 1, 01.01.1995, p. 41-47.

Research output: Contribution to journalArticle

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AU - Tanaka, Nobuo

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AU - Barnhart, Elizabeth R.

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