Sample-to-answer palm-sized nucleic acid testing device towards low-cost malaria mass screening

Gihoon Choi, Theodore Prince, Jun Miao, Liwang Cui, Weihua Guan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The effectiveness of malaria screening and treatment highly depends on the low-cost access to the highly sensitive and specific malaria test. We report a real-time fluorescence nucleic acid testing device for malaria field detection with automated and scalable sample preparation capability. The device consists a compact analyzer and a disposable microfluidic reagent compact disc. The parasite DNA sample preparation and subsequent real-time LAMP detection were seamlessly integrated on a single microfluidic compact disc, driven by energy efficient non-centrifuge based magnetic field interactions. Each disc contains four parallel testing units which could be configured either as four identical tests or as four species-specific tests. When configured as species-specific tests, it could identify two of the most life-threatening malaria species (P. falciparum and P. vivax). The NAT device is capable of processing four samples simultaneously within 50 min turnaround time. It achieves a detection limit of ~0.5 parasites/µl for whole blood, sufficient for detecting asymptomatic parasite carriers. The combination of the sensitivity, specificity, cost, and scalable sample preparation suggests the real-time fluorescence LAMP device could be particularly useful for malaria screening in the field settings.

Original languageEnglish (US)
Pages (from-to)83-90
Number of pages8
JournalBiosensors and Bioelectronics
Volume115
DOIs
StatePublished - Sep 15 2018

Fingerprint

Mass Screening
Nucleic acids
Nucleic Acids
Malaria
Screening
Microfluidics
Costs and Cost Analysis
Equipment and Supplies
Testing
Fluorescence
Parasites
Costs
Turnaround time
DNA
Blood
Magnetic fields
Magnetic Fields
Limit of Detection
Processing
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Choi, Gihoon ; Prince, Theodore ; Miao, Jun ; Cui, Liwang ; Guan, Weihua. / Sample-to-answer palm-sized nucleic acid testing device towards low-cost malaria mass screening. In: Biosensors and Bioelectronics. 2018 ; Vol. 115. pp. 83-90.
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Sample-to-answer palm-sized nucleic acid testing device towards low-cost malaria mass screening. / Choi, Gihoon; Prince, Theodore; Miao, Jun; Cui, Liwang; Guan, Weihua.

In: Biosensors and Bioelectronics, Vol. 115, 15.09.2018, p. 83-90.

Research output: Contribution to journalArticle

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