Scaffolds from alternating block polyurethanes of poly(ε-caprolactone) and poly(ethylene glycol) with stimulation and guidance of nerve growth and better nerve repair than autograft

Yuqing Niu, Linjing Li, Kevin C. Chen, Feiran Chen, Xiangyu Liu, Jianfu Ye, Wei Li, Kaitian Xu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Abstract Nerve repair scaffolds from novel alternating block polyurethanes (PUCL-alt-PEG) based on PCL and PEG without additional growth factors or proteins were prepared by a particle leaching method. The scaffolds have pore size 10-20 μm and porosity 92%. Mechanical tests showed that the polyurethane scaffolds have maximum loads of 5.97±0.35 N and maximal stresses of 8.84±0.5 MPa. Histocompatiblity of the nerve repair scaffolds was tested in a SD rat model for peripheral nerve defect treatment. Two types of treatments including PUCL-alt-PEG scaffolds and autografts were compared in rat model. After 32 weeks, bridging of a 12 mm defect gap by the regenerated nerve was observed in all rats. The nerve regeneration was systematically characterized by sciatic function index (SFI), electrophysiology, histological assessment including HE staining, immunohistochemistry, ammonia sliver staining, Masson's trichrome staining and TEM observation. Results revealed that nerve repair scaffolds from PUCL-alt-PEG exhibit better regeneration effects compared to autografts. Electrophysiological recovery was seen in 90% and 87% of rats in PUCL-alt-PEG and autograft groups respectively. Biodegradation in vitro and in vivo shows good degradation match of PUCL-alt-PEG scaffolds with nerve regeneration. It demonstrates that plain nerve repair scaffolds from PUCL-alt-PEG biomaterials can achieve peripheral nerve regeneration satisfactorily.

Original languageEnglish (US)
Pages (from-to)2355-2364
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume103
Issue number7
DOIs
StatePublished - Jul 1 2015

Fingerprint

Polyurethanes
Autografts
Scaffolds
Polyethylene glycols
Repair
Rats
Electrophysiology
Defects
polycaprolactone
Biocompatible Materials
Biodegradation
Ammonia
Biomaterials
Leaching
Pore size
Loads (forces)
Intercellular Signaling Peptides and Proteins
Porosity
Transmission electron microscopy
Proteins

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Niu, Yuqing ; Li, Linjing ; Chen, Kevin C. ; Chen, Feiran ; Liu, Xiangyu ; Ye, Jianfu ; Li, Wei ; Xu, Kaitian. / Scaffolds from alternating block polyurethanes of poly(ε-caprolactone) and poly(ethylene glycol) with stimulation and guidance of nerve growth and better nerve repair than autograft. In: Journal of Biomedical Materials Research - Part A. 2015 ; Vol. 103, No. 7. pp. 2355-2364.
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abstract = "Abstract Nerve repair scaffolds from novel alternating block polyurethanes (PUCL-alt-PEG) based on PCL and PEG without additional growth factors or proteins were prepared by a particle leaching method. The scaffolds have pore size 10-20 μm and porosity 92{\%}. Mechanical tests showed that the polyurethane scaffolds have maximum loads of 5.97±0.35 N and maximal stresses of 8.84±0.5 MPa. Histocompatiblity of the nerve repair scaffolds was tested in a SD rat model for peripheral nerve defect treatment. Two types of treatments including PUCL-alt-PEG scaffolds and autografts were compared in rat model. After 32 weeks, bridging of a 12 mm defect gap by the regenerated nerve was observed in all rats. The nerve regeneration was systematically characterized by sciatic function index (SFI), electrophysiology, histological assessment including HE staining, immunohistochemistry, ammonia sliver staining, Masson's trichrome staining and TEM observation. Results revealed that nerve repair scaffolds from PUCL-alt-PEG exhibit better regeneration effects compared to autografts. Electrophysiological recovery was seen in 90{\%} and 87{\%} of rats in PUCL-alt-PEG and autograft groups respectively. Biodegradation in vitro and in vivo shows good degradation match of PUCL-alt-PEG scaffolds with nerve regeneration. It demonstrates that plain nerve repair scaffolds from PUCL-alt-PEG biomaterials can achieve peripheral nerve regeneration satisfactorily.",
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Scaffolds from alternating block polyurethanes of poly(ε-caprolactone) and poly(ethylene glycol) with stimulation and guidance of nerve growth and better nerve repair than autograft. / Niu, Yuqing; Li, Linjing; Chen, Kevin C.; Chen, Feiran; Liu, Xiangyu; Ye, Jianfu; Li, Wei; Xu, Kaitian.

In: Journal of Biomedical Materials Research - Part A, Vol. 103, No. 7, 01.07.2015, p. 2355-2364.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Scaffolds from alternating block polyurethanes of poly(ε-caprolactone) and poly(ethylene glycol) with stimulation and guidance of nerve growth and better nerve repair than autograft

AU - Niu, Yuqing

AU - Li, Linjing

AU - Chen, Kevin C.

AU - Chen, Feiran

AU - Liu, Xiangyu

AU - Ye, Jianfu

AU - Li, Wei

AU - Xu, Kaitian

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N2 - Abstract Nerve repair scaffolds from novel alternating block polyurethanes (PUCL-alt-PEG) based on PCL and PEG without additional growth factors or proteins were prepared by a particle leaching method. The scaffolds have pore size 10-20 μm and porosity 92%. Mechanical tests showed that the polyurethane scaffolds have maximum loads of 5.97±0.35 N and maximal stresses of 8.84±0.5 MPa. Histocompatiblity of the nerve repair scaffolds was tested in a SD rat model for peripheral nerve defect treatment. Two types of treatments including PUCL-alt-PEG scaffolds and autografts were compared in rat model. After 32 weeks, bridging of a 12 mm defect gap by the regenerated nerve was observed in all rats. The nerve regeneration was systematically characterized by sciatic function index (SFI), electrophysiology, histological assessment including HE staining, immunohistochemistry, ammonia sliver staining, Masson's trichrome staining and TEM observation. Results revealed that nerve repair scaffolds from PUCL-alt-PEG exhibit better regeneration effects compared to autografts. Electrophysiological recovery was seen in 90% and 87% of rats in PUCL-alt-PEG and autograft groups respectively. Biodegradation in vitro and in vivo shows good degradation match of PUCL-alt-PEG scaffolds with nerve regeneration. It demonstrates that plain nerve repair scaffolds from PUCL-alt-PEG biomaterials can achieve peripheral nerve regeneration satisfactorily.

AB - Abstract Nerve repair scaffolds from novel alternating block polyurethanes (PUCL-alt-PEG) based on PCL and PEG without additional growth factors or proteins were prepared by a particle leaching method. The scaffolds have pore size 10-20 μm and porosity 92%. Mechanical tests showed that the polyurethane scaffolds have maximum loads of 5.97±0.35 N and maximal stresses of 8.84±0.5 MPa. Histocompatiblity of the nerve repair scaffolds was tested in a SD rat model for peripheral nerve defect treatment. Two types of treatments including PUCL-alt-PEG scaffolds and autografts were compared in rat model. After 32 weeks, bridging of a 12 mm defect gap by the regenerated nerve was observed in all rats. The nerve regeneration was systematically characterized by sciatic function index (SFI), electrophysiology, histological assessment including HE staining, immunohistochemistry, ammonia sliver staining, Masson's trichrome staining and TEM observation. Results revealed that nerve repair scaffolds from PUCL-alt-PEG exhibit better regeneration effects compared to autografts. Electrophysiological recovery was seen in 90% and 87% of rats in PUCL-alt-PEG and autograft groups respectively. Biodegradation in vitro and in vivo shows good degradation match of PUCL-alt-PEG scaffolds with nerve regeneration. It demonstrates that plain nerve repair scaffolds from PUCL-alt-PEG biomaterials can achieve peripheral nerve regeneration satisfactorily.

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