TY - JOUR
T1 - Schizosaccharomyces pombe Rad9 contains a BH3-like region and interacts with the anti-apoptotic protein Bcl-2
AU - Komatsu, Kiyoshi
AU - Hopkins, Kevin M.
AU - Lieberman, Howard B.
AU - Wang, Hong Gang
PY - 2000/9/15
Y1 - 2000/9/15
N2 - Here we report that the Schizosaccharomyces pombe Rad9 (SpRad9) protein contains a group of amino acids with similarity to the Bcl-2 homology 3 death domain, which is required for SpRad9 interaction with human Bcl-2 and apoptosis induction in human cells. Overexpression of Bcl-2 in S. pombe inhibits cell growth independently of rad9, but enhances resistance of rad9-null cells to methyl methanesulfonate, ultraviolet and ionizing radiation. These observations suggest that SpRad9 may represent the first member of the Bcl-2 protein family identified in yeast, though the cell death pathways in S. pombe may differ from those found in mammals. (C) 2000 Federation of European Biochemical Societies.
AB - Here we report that the Schizosaccharomyces pombe Rad9 (SpRad9) protein contains a group of amino acids with similarity to the Bcl-2 homology 3 death domain, which is required for SpRad9 interaction with human Bcl-2 and apoptosis induction in human cells. Overexpression of Bcl-2 in S. pombe inhibits cell growth independently of rad9, but enhances resistance of rad9-null cells to methyl methanesulfonate, ultraviolet and ionizing radiation. These observations suggest that SpRad9 may represent the first member of the Bcl-2 protein family identified in yeast, though the cell death pathways in S. pombe may differ from those found in mammals. (C) 2000 Federation of European Biochemical Societies.
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U2 - 10.1016/S0014-5793(00)01975-X
DO - 10.1016/S0014-5793(00)01975-X
M3 - Article
C2 - 10996309
AN - SCOPUS:0034666388
SN - 0014-5793
VL - 481
SP - 122
EP - 126
JO - FEBS Letters
JF - FEBS Letters
IS - 2
ER -