Scintillation proximity assay for total p53 protein as an alternative to ELISA

Sarit Oberlander, Tian Xie, Uma Chandrachud, Susannah Gal

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Measurement of the level of a specific protein can be an important parameter to discern as that can change and reflect disease status. A number of methods have been developed to quantitate the level of a protein, some amenable to high throughput screening. A method is described to measure the total level of the tumor suppressor p53 using scintillation proximity assay (SPA) beads and radiolabeled streptavidin. Three different cell extracts were used, with one used to develop the standard curve for the amount of p53. This method allows the specific detection of p53 in the range of 50 to 300. pg in 10 μl of an extract. While this detection is less than what can be detected by commercially available enzyme linked immunosorbent assay (ELISA) kits, the SPA compares favorably on time required and cost. This new assay also has the potential to be coupled with measurements for p53 DNA binding, a unique aspect of this approach.

Original languageEnglish (US)
Pages (from-to)173-177
Number of pages5
JournalJournal of Immunological Methods
Volume360
Issue number1-2
DOIs
StatePublished - Aug 2010

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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