Scleraxis expression is coordinately regulated in a murine model of patellar tendon injury

Alexander Scott, Arthur Sampaio, Thomas Abraham, Chris Duronio, Tully M. Underhill

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

This study investigated the expression of Scleraxis in a murine model of patellar tendon injury in which the central third of the patellar tendon was unilaterally injured. The presence of tendon pathology was assessed using dual photon microscopy, conventional histology and microCT. Tendon pathology was also quantified noninvasively over a 12-week period using high-frequency ultrasound and laser Doppler flowmetry. Gene expression (Scx, Tnmd, and Col1a1) was determined at defined end-points (1, 4, 8, and 12 weeks) using qPCR on RNA from individual patellar tendons on injured and uninjured sides. There was significant development of tendon pathology as gauged by ultrasound and laser Doppler over 12 weeks. Injured tendons demonstrated significant histological and microCT evidence of pathological change, and disorganized collagen with reduced density. The expression of Scx and Col1a1 was unchanged at 1 week, significantly upregulated at 4 and 8 weeks, and had returned to baseline by 12 weeks. Tnmd expression was unchanged at 1 week, and significantly increased at 4, 8, and 12 weeks. Patellar tendon injury was associated with marked increases in the expression of Scx, Tnmd, and Col1a1. Our data suggest new roles for Scleraxis in coordinating the response to injury in the pathogenesis of tendon disorders.

Original languageEnglish (US)
Pages (from-to)289-296
Number of pages8
JournalJournal of Orthopaedic Research
Volume29
Issue number2
DOIs
StatePublished - Feb 1 2011

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Tendon Injuries
Patellar Ligament
Tendons
X-Ray Microtomography
Pathology
Doppler Ultrasonography
Laser-Doppler Flowmetry
Photons
Microscopy
Histology
Lasers
Collagen
RNA
Gene Expression
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine

Cite this

Scott, Alexander ; Sampaio, Arthur ; Abraham, Thomas ; Duronio, Chris ; Underhill, Tully M. / Scleraxis expression is coordinately regulated in a murine model of patellar tendon injury. In: Journal of Orthopaedic Research. 2011 ; Vol. 29, No. 2. pp. 289-296.
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Scleraxis expression is coordinately regulated in a murine model of patellar tendon injury. / Scott, Alexander; Sampaio, Arthur; Abraham, Thomas; Duronio, Chris; Underhill, Tully M.

In: Journal of Orthopaedic Research, Vol. 29, No. 2, 01.02.2011, p. 289-296.

Research output: Contribution to journalArticle

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