Screening for cellulose nanowhiskers binding peptides by phage display

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cellulose nanowhiskers (CNWs) are the crystalline portion of cellulose fibers which can be extracted through acid hydrolysis process from cotton. Phage display technique was used to screen for small peptide ligands that selectively bind to the CNWs from a library of approximately 1 billion different polypeptides. These peptides were selected on immobilized CNWs by bio-panning, by which a pool of randomized peptides were screened based on the binding affinity to the target CNWs. After three round of bio-panning, a consensus peptide sequence of "WHWTYYW" was found to efficiently bind to CNWs. The binding between the "WHWTYYW"-harboring phage and CNWs was confirmed by single phage bio-panning assay and the binding affinity was further characterized by Ultraviolet Difference Spectroscopy.

Original languageEnglish (US)
Title of host publicationAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010
PublisherAmerican Society of Agricultural and Biological Engineers
Pages5137-5149
Number of pages13
Volume6
ISBN (Print)9781617388354
StatePublished - 2010

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Cellulose
bacteriophages
Bacteriophages
cellulose
peptides
screening
Peptides
Cell Surface Display Techniques
cellulosic fibers
acid hydrolysis
Consensus Sequence
spectroscopy
polypeptides
cotton
Spectrum Analysis
Hydrolysis
Ligands
assays
Acids

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Guo, J., Catchmark, J. M., Tien, M., & Kao, T. (2010). Screening for cellulose nanowhiskers binding peptides by phage display. In American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010 (Vol. 6, pp. 5137-5149). American Society of Agricultural and Biological Engineers.
Guo, Jing ; Catchmark, Jeffrey M. ; Tien, Ming ; Kao, Teh-hui. / Screening for cellulose nanowhiskers binding peptides by phage display. American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010. Vol. 6 American Society of Agricultural and Biological Engineers, 2010. pp. 5137-5149
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abstract = "Cellulose nanowhiskers (CNWs) are the crystalline portion of cellulose fibers which can be extracted through acid hydrolysis process from cotton. Phage display technique was used to screen for small peptide ligands that selectively bind to the CNWs from a library of approximately 1 billion different polypeptides. These peptides were selected on immobilized CNWs by bio-panning, by which a pool of randomized peptides were screened based on the binding affinity to the target CNWs. After three round of bio-panning, a consensus peptide sequence of {"}WHWTYYW{"} was found to efficiently bind to CNWs. The binding between the {"}WHWTYYW{"}-harboring phage and CNWs was confirmed by single phage bio-panning assay and the binding affinity was further characterized by Ultraviolet Difference Spectroscopy.",
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Guo, J, Catchmark, JM, Tien, M & Kao, T 2010, Screening for cellulose nanowhiskers binding peptides by phage display. in American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010. vol. 6, American Society of Agricultural and Biological Engineers, pp. 5137-5149.

Screening for cellulose nanowhiskers binding peptides by phage display. / Guo, Jing; Catchmark, Jeffrey M.; Tien, Ming; Kao, Teh-hui.

American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010. Vol. 6 American Society of Agricultural and Biological Engineers, 2010. p. 5137-5149.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - Screening for cellulose nanowhiskers binding peptides by phage display

AU - Guo, Jing

AU - Catchmark, Jeffrey M.

AU - Tien, Ming

AU - Kao, Teh-hui

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N2 - Cellulose nanowhiskers (CNWs) are the crystalline portion of cellulose fibers which can be extracted through acid hydrolysis process from cotton. Phage display technique was used to screen for small peptide ligands that selectively bind to the CNWs from a library of approximately 1 billion different polypeptides. These peptides were selected on immobilized CNWs by bio-panning, by which a pool of randomized peptides were screened based on the binding affinity to the target CNWs. After three round of bio-panning, a consensus peptide sequence of "WHWTYYW" was found to efficiently bind to CNWs. The binding between the "WHWTYYW"-harboring phage and CNWs was confirmed by single phage bio-panning assay and the binding affinity was further characterized by Ultraviolet Difference Spectroscopy.

AB - Cellulose nanowhiskers (CNWs) are the crystalline portion of cellulose fibers which can be extracted through acid hydrolysis process from cotton. Phage display technique was used to screen for small peptide ligands that selectively bind to the CNWs from a library of approximately 1 billion different polypeptides. These peptides were selected on immobilized CNWs by bio-panning, by which a pool of randomized peptides were screened based on the binding affinity to the target CNWs. After three round of bio-panning, a consensus peptide sequence of "WHWTYYW" was found to efficiently bind to CNWs. The binding between the "WHWTYYW"-harboring phage and CNWs was confirmed by single phage bio-panning assay and the binding affinity was further characterized by Ultraviolet Difference Spectroscopy.

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Guo J, Catchmark JM, Tien M, Kao T. Screening for cellulose nanowhiskers binding peptides by phage display. In American Society of Agricultural and Biological Engineers Annual International Meeting 2010, ASABE 2010. Vol. 6. American Society of Agricultural and Biological Engineers. 2010. p. 5137-5149