Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies

Masayuki Takahashi, Reyad A. Elbarbary, Norihiro Watanabe, Atsushi Goto, Daichi Kamiya, Yoshihiro Watabe, Takayoshi Uchiyama, Miwako Narita, Masuhiro Takahashi, Yoshiaki Takahashi, Noriko Ishihara, Tatsuya Miyazawa, Tetsuo Yoshida, Mitsuoki Kawano, Masato Tamura, Masayuki Nashimoto

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

tRNase-ZL-utilizing efficacious (TRUE) gene silencing is an RNA-mediated gene expression control technology that has therapeutic potential. This technology is based on the property of tRNase ZL that it can cleave any target RNA at any desired site under the direction of an appropriate artificial small guide RNA (sgRNA). To search for novel potential therapeutic sgRNAs for hematological malignancies, we screened a library composed of 156 sgRNAs, and found that 20 sgRNAs can efficiently induce apoptosis in leukemia and/or myeloma cells. Furthermore, we demonstrated that 4 of the 20 sgRNAs can reduce growth rates of HL60 cells in mouse xenograft models.

Original languageEnglish (US)
Pages (from-to)808-815
Number of pages8
JournalLeukemia Research
Volume38
Issue number7
DOIs
StatePublished - Jul 2014

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

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    Takahashi, M., Elbarbary, R. A., Watanabe, N., Goto, A., Kamiya, D., Watabe, Y., Uchiyama, T., Narita, M., Takahashi, M., Takahashi, Y., Ishihara, N., Miyazawa, T., Yoshida, T., Kawano, M., Tamura, M., & Nashimoto, M. (2014). Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies. Leukemia Research, 38(7), 808-815. https://doi.org/10.1016/j.leukres.2014.03.021