Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies

Masayuki Takahashi, Reyad A. Elbarbary, Norihiro Watanabe, Atsushi Goto, Daichi Kamiya, Yoshihiro Watabe, Takayoshi Uchiyama, Miwako Narita, Masuhiro Takahashi, Yoshiaki Takahashi, Noriko Ishihara, Tatsuya Miyazawa, Tetsuo Yoshida, Mitsuoki Kawano, Masato Tamura, Masayuki Nashimoto

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

tRNase-ZL-utilizing efficacious (TRUE) gene silencing is an RNA-mediated gene expression control technology that has therapeutic potential. This technology is based on the property of tRNase ZL that it can cleave any target RNA at any desired site under the direction of an appropriate artificial small guide RNA (sgRNA). To search for novel potential therapeutic sgRNAs for hematological malignancies, we screened a library composed of 156 sgRNAs, and found that 20 sgRNAs can efficiently induce apoptosis in leukemia and/or myeloma cells. Furthermore, we demonstrated that 4 of the 20 sgRNAs can reduce growth rates of HL60 cells in mouse xenograft models.

Original languageEnglish (US)
Pages (from-to)808-815
Number of pages8
JournalLeukemia Research
Volume38
Issue number7
DOIs
StatePublished - Jul 2014

Fingerprint

Guide RNA
Hematologic Neoplasms
Libraries
RNA
Technology
HL-60 Cells
Gene Silencing
Heterografts
Leukemia
Apoptosis
Gene Expression
Therapeutics
Growth

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Takahashi, Masayuki ; Elbarbary, Reyad A. ; Watanabe, Norihiro ; Goto, Atsushi ; Kamiya, Daichi ; Watabe, Yoshihiro ; Uchiyama, Takayoshi ; Narita, Miwako ; Takahashi, Masuhiro ; Takahashi, Yoshiaki ; Ishihara, Noriko ; Miyazawa, Tatsuya ; Yoshida, Tetsuo ; Kawano, Mitsuoki ; Tamura, Masato ; Nashimoto, Masayuki. / Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies. In: Leukemia Research. 2014 ; Vol. 38, No. 7. pp. 808-815.
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abstract = "tRNase-ZL-utilizing efficacious (TRUE) gene silencing is an RNA-mediated gene expression control technology that has therapeutic potential. This technology is based on the property of tRNase ZL that it can cleave any target RNA at any desired site under the direction of an appropriate artificial small guide RNA (sgRNA). To search for novel potential therapeutic sgRNAs for hematological malignancies, we screened a library composed of 156 sgRNAs, and found that 20 sgRNAs can efficiently induce apoptosis in leukemia and/or myeloma cells. Furthermore, we demonstrated that 4 of the 20 sgRNAs can reduce growth rates of HL60 cells in mouse xenograft models.",
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Takahashi, M, Elbarbary, RA, Watanabe, N, Goto, A, Kamiya, D, Watabe, Y, Uchiyama, T, Narita, M, Takahashi, M, Takahashi, Y, Ishihara, N, Miyazawa, T, Yoshida, T, Kawano, M, Tamura, M & Nashimoto, M 2014, 'Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies', Leukemia Research, vol. 38, no. 7, pp. 808-815. https://doi.org/10.1016/j.leukres.2014.03.021

Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies. / Takahashi, Masayuki; Elbarbary, Reyad A.; Watanabe, Norihiro; Goto, Atsushi; Kamiya, Daichi; Watabe, Yoshihiro; Uchiyama, Takayoshi; Narita, Miwako; Takahashi, Masuhiro; Takahashi, Yoshiaki; Ishihara, Noriko; Miyazawa, Tatsuya; Yoshida, Tetsuo; Kawano, Mitsuoki; Tamura, Masato; Nashimoto, Masayuki.

In: Leukemia Research, Vol. 38, No. 7, 07.2014, p. 808-815.

Research output: Contribution to journalArticle

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T1 - Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies

AU - Takahashi, Masayuki

AU - Elbarbary, Reyad A.

AU - Watanabe, Norihiro

AU - Goto, Atsushi

AU - Kamiya, Daichi

AU - Watabe, Yoshihiro

AU - Uchiyama, Takayoshi

AU - Narita, Miwako

AU - Takahashi, Masuhiro

AU - Takahashi, Yoshiaki

AU - Ishihara, Noriko

AU - Miyazawa, Tatsuya

AU - Yoshida, Tetsuo

AU - Kawano, Mitsuoki

AU - Tamura, Masato

AU - Nashimoto, Masayuki

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