Second messenger-mediated spatiotemporal control of protein degradation regulates bacterial cell cycle progression

Anna Duerig, Sören Abel, Marc Folcher, Micael Nicollier, Torsten Schwede, Nicolas Amiot, Bernd Giese, Urs Jenal

Research output: Contribution to journalArticlepeer-review

202 Scopus citations

Abstract

Second messengers control a wide range of important cellular functions in eukaryotes and prokaryotes. Here we show that cyclic di-GMP, a global bacterial second messenger, promotes cell cycle progression in Caulobacter crescentus by mediating the specific degradation of the replication initiation inhibitor CtrA. During the G1-to-S-phase transition, both CtrA and its cognate protease ClpXP dynamically localize to the old cell pole, where CtrA is rapidly degraded. Sequestration of CtrA to the cell pole depends on PopA, a newly identified cyclic di-GMP effector protein. PopA itself localizes to the cell pole and directs CtrA to this subcellular site via the direct interaction with a mediator protein, RcdA. We present evidence that c-di-GMP regulates CtrA degradation during the cell cycle by controlling the dynamic sequestration of the PopA recruitment factor to the cell pole. Furthermore, we show that cell cycle timing of CtrA degradation relies on converging pathways responsible for substrate and protease localization to the old cell pole. This is the first report that links cyclic di-GMP to protein dynamics and cell cycle control in bacteria.

Original languageEnglish (US)
Pages (from-to)93-104
Number of pages12
JournalGenes and Development
Volume23
Issue number1
DOIs
StatePublished - Jan 1 2009

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

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