Secondary-Ion Mass Spectrometry Images Cardiolipins and Phosphatidylethanolamines at the Subcellular Level

Hua Tian, Louis J. Sparvero, Paul Blenkinsopp, Andrew A. Amoscato, Simon C. Watkins, Hülya Bayır, Valerian E. Kagan, Nicholas Winograd

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Millions of diverse molecules constituting the lipidome act as important signals within cells. Of these, cardiolipin (CL) and phosphatidylethanolamine (PE) participate in apoptosis and ferroptosis, respectively. Their subcellular distribution is largely unknown. Imaging mass spectrometry is capable of deciphering the spatial distribution of multiple lipids at subcellular levels. Here we report the development of a unique 70 keV gas-cluster ion beam that consists of (CO 2 ) n + (n>10 000) projectiles. Coupled with direct current beam buncher-time-of-flight secondary-ion mass spectrometry, it is optimized for sensitivity towards high-mass species (up to m/z 3000) at high spatial resolution (1 μm). In combination with immunohistochemistry, phospholipids, including PE and CL, have been assessed in subcellular compartments of mouse hippocampal neuronal cells and rat brain tissue.

Original languageEnglish (US)
Pages (from-to)3156-3161
Number of pages6
JournalAngewandte Chemie - International Edition
Volume58
Issue number10
DOIs
StatePublished - Mar 4 2019

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

Fingerprint Dive into the research topics of 'Secondary-Ion Mass Spectrometry Images Cardiolipins and Phosphatidylethanolamines at the Subcellular Level'. Together they form a unique fingerprint.

Cite this