Segregation of random amplified DNA markers in F1 progeny of conifers

John Edward Carlson, L. K. Tulsieram, J. C. Glaubitz, V. W.K. Luk, C. Kauffeldt, R. Rutledge

Research output: Contribution to journalArticle

258 Citations (Scopus)

Abstract

The recently developed approach to deriving genetic markers via amplification of random DNA segments with single primers of arbitrary nucleotide sequence was tested for its utility in genetic linkage mapping studies with conifers. Reaction conditions were optimized to reproducibly yield clean and specific amplification products. Template DNA from several genotypes of Douglas-fir (Pseudotsuga menziesii) and white spruce (Picea glauca) were tested against eight ten-base oligonucleotide primers. Most of the tested primer/parent tree combinations yielded polymorphic PCR products ("RAPD" markers). Selected primers were then used in PCR reactions with template DNA isolated from offspring in Douglas-fir and black spruce diallel crosses among the same parental lines. The diallel study confirmed the appropriate inheritance of RAPD markers in the F1 generation. The value of these dominant RAPD markers for genetic linkage mapping in trees was established from both theoretical and applied perspectives.

Original languageEnglish (US)
Pages (from-to)194-200
Number of pages7
JournalTheoretical And Applied Genetics
Volume83
Issue number2
DOIs
StatePublished - Dec 1 1991

Fingerprint

Pseudotsuga
Coniferophyta
Pseudotsuga menziesii
Genetic Markers
conifers
Genetic Linkage
Picea glauca
Chromosome Mapping
genetic markers
chromosome mapping
DNA
Picea
Polymerase Chain Reaction
DNA Primers
diallel analysis
Picea mariana
DNA primers
inheritance (genetics)
Genotype
nucleotide sequences

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

Carlson, J. E., Tulsieram, L. K., Glaubitz, J. C., Luk, V. W. K., Kauffeldt, C., & Rutledge, R. (1991). Segregation of random amplified DNA markers in F1 progeny of conifers. Theoretical And Applied Genetics, 83(2), 194-200. https://doi.org/10.1007/BF00226251
Carlson, John Edward ; Tulsieram, L. K. ; Glaubitz, J. C. ; Luk, V. W.K. ; Kauffeldt, C. ; Rutledge, R. / Segregation of random amplified DNA markers in F1 progeny of conifers. In: Theoretical And Applied Genetics. 1991 ; Vol. 83, No. 2. pp. 194-200.
@article{12b6249587534f4591ca3c33fc6a8545,
title = "Segregation of random amplified DNA markers in F1 progeny of conifers",
abstract = "The recently developed approach to deriving genetic markers via amplification of random DNA segments with single primers of arbitrary nucleotide sequence was tested for its utility in genetic linkage mapping studies with conifers. Reaction conditions were optimized to reproducibly yield clean and specific amplification products. Template DNA from several genotypes of Douglas-fir (Pseudotsuga menziesii) and white spruce (Picea glauca) were tested against eight ten-base oligonucleotide primers. Most of the tested primer/parent tree combinations yielded polymorphic PCR products ({"}RAPD{"} markers). Selected primers were then used in PCR reactions with template DNA isolated from offspring in Douglas-fir and black spruce diallel crosses among the same parental lines. The diallel study confirmed the appropriate inheritance of RAPD markers in the F1 generation. The value of these dominant RAPD markers for genetic linkage mapping in trees was established from both theoretical and applied perspectives.",
author = "Carlson, {John Edward} and Tulsieram, {L. K.} and Glaubitz, {J. C.} and Luk, {V. W.K.} and C. Kauffeldt and R. Rutledge",
year = "1991",
month = "12",
day = "1",
doi = "10.1007/BF00226251",
language = "English (US)",
volume = "83",
pages = "194--200",
journal = "Theoretical And Applied Genetics",
issn = "0040-5752",
publisher = "Springer Verlag",
number = "2",

}

Carlson, JE, Tulsieram, LK, Glaubitz, JC, Luk, VWK, Kauffeldt, C & Rutledge, R 1991, 'Segregation of random amplified DNA markers in F1 progeny of conifers', Theoretical And Applied Genetics, vol. 83, no. 2, pp. 194-200. https://doi.org/10.1007/BF00226251

Segregation of random amplified DNA markers in F1 progeny of conifers. / Carlson, John Edward; Tulsieram, L. K.; Glaubitz, J. C.; Luk, V. W.K.; Kauffeldt, C.; Rutledge, R.

In: Theoretical And Applied Genetics, Vol. 83, No. 2, 01.12.1991, p. 194-200.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Segregation of random amplified DNA markers in F1 progeny of conifers

AU - Carlson, John Edward

AU - Tulsieram, L. K.

AU - Glaubitz, J. C.

AU - Luk, V. W.K.

AU - Kauffeldt, C.

AU - Rutledge, R.

PY - 1991/12/1

Y1 - 1991/12/1

N2 - The recently developed approach to deriving genetic markers via amplification of random DNA segments with single primers of arbitrary nucleotide sequence was tested for its utility in genetic linkage mapping studies with conifers. Reaction conditions were optimized to reproducibly yield clean and specific amplification products. Template DNA from several genotypes of Douglas-fir (Pseudotsuga menziesii) and white spruce (Picea glauca) were tested against eight ten-base oligonucleotide primers. Most of the tested primer/parent tree combinations yielded polymorphic PCR products ("RAPD" markers). Selected primers were then used in PCR reactions with template DNA isolated from offspring in Douglas-fir and black spruce diallel crosses among the same parental lines. The diallel study confirmed the appropriate inheritance of RAPD markers in the F1 generation. The value of these dominant RAPD markers for genetic linkage mapping in trees was established from both theoretical and applied perspectives.

AB - The recently developed approach to deriving genetic markers via amplification of random DNA segments with single primers of arbitrary nucleotide sequence was tested for its utility in genetic linkage mapping studies with conifers. Reaction conditions were optimized to reproducibly yield clean and specific amplification products. Template DNA from several genotypes of Douglas-fir (Pseudotsuga menziesii) and white spruce (Picea glauca) were tested against eight ten-base oligonucleotide primers. Most of the tested primer/parent tree combinations yielded polymorphic PCR products ("RAPD" markers). Selected primers were then used in PCR reactions with template DNA isolated from offspring in Douglas-fir and black spruce diallel crosses among the same parental lines. The diallel study confirmed the appropriate inheritance of RAPD markers in the F1 generation. The value of these dominant RAPD markers for genetic linkage mapping in trees was established from both theoretical and applied perspectives.

UR - http://www.scopus.com/inward/record.url?scp=33749781112&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749781112&partnerID=8YFLogxK

U2 - 10.1007/BF00226251

DO - 10.1007/BF00226251

M3 - Article

C2 - 24202358

AN - SCOPUS:33749781112

VL - 83

SP - 194

EP - 200

JO - Theoretical And Applied Genetics

JF - Theoretical And Applied Genetics

SN - 0040-5752

IS - 2

ER -