Seizure entrainment with polarizing low-frequency electric fields in a chronic animal epilepsy model

Sridhar Sunderam, Nick Chernyy, Nathalia Peixoto, Jonathan P. Mason, Steven L. Weinstein, Steven J. Schiff, Bruce J. Gluckman

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Neural activity can be modulated by applying a polarizing low-frequency (≪100 Hz) electric field (PLEF). Unlike conventional pulsed stimulation, PLEF stimulation has a graded, modulatory effect on neuronal excitability, and permits the simultaneous recording of neuronal activity during stimulation suitable for continuous feedback control. We tested a prototype system that allows for simultaneous PLEF stimulation with minimal recording artifact in a chronic tetanus toxin animal model (rat) of hippocampal epilepsy with spontaneous seizures. Depth electrode local field potentials recorded during seizures revealed a characteristic pattern of field postsynaptic potentials (fPSPs). Sinusoidal voltage-controlled PLEF stimulation (0.5-25 Hz) was applied in open-loop cycles radially across the CA3 of ventral hippocampus. For stimulated seizures, fPSPs were transiently entrained with the PLEF waveform. Statistical significance of entrainment was assessed with Thomson's harmonic F-test, with 45/132 stimulated seizures in four animals individually demonstrating significant entrainment (p < 0.04). Significant entrainment for multiple presentations at the same frequency (p < 0.01) was observed in three of four animals in 42/64 stimulated seizures. This is the first demonstration in chronically implanted freely behaving animals of PLEF modulation of neural activity with simultaneous recording.

Original languageEnglish (US)
Article number046009
JournalJournal of neural engineering
Volume6
Issue number4
DOIs
StatePublished - Nov 24 2009

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Epilepsy
Animals
Seizures
Animal Models
Electric fields
Synaptic Potentials
Tetanus Toxin
Feedback control
Rats
Demonstrations
Artifacts
Modulation
Hippocampus
Electrodes
Electric potential

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Cellular and Molecular Neuroscience

Cite this

Sunderam, Sridhar ; Chernyy, Nick ; Peixoto, Nathalia ; Mason, Jonathan P. ; Weinstein, Steven L. ; Schiff, Steven J. ; Gluckman, Bruce J. / Seizure entrainment with polarizing low-frequency electric fields in a chronic animal epilepsy model. In: Journal of neural engineering. 2009 ; Vol. 6, No. 4.
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Seizure entrainment with polarizing low-frequency electric fields in a chronic animal epilepsy model. / Sunderam, Sridhar; Chernyy, Nick; Peixoto, Nathalia; Mason, Jonathan P.; Weinstein, Steven L.; Schiff, Steven J.; Gluckman, Bruce J.

In: Journal of neural engineering, Vol. 6, No. 4, 046009, 24.11.2009.

Research output: Contribution to journalArticle

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