Selection and screening strategies

Haiying Liang, P. Ananda Kumar, Vikrant Nain, William A. Powell, John Edward Carlson

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

A number of transformation systems have been developed to insert foreign DNA into the appropriate plant genome (nuclear or plastid) (discussed in Chap. 3). However, only a small fraction of the treated cells become transgenic, while the majority of the cells remain untransformed using any of these methods. Thus, effective selection and screening strategies are needed to pick up the rare transgenic lines from a pool of nontransformed cells or plants. To date, more than 50 marker genes and a few molecular techniques have been developed to serve this essential purpose.

Original languageEnglish (US)
Title of host publicationPrinciples and Development
PublisherSpringer-Verlag Berlin Heidelberg
Pages85-143
Number of pages59
Volume1
ISBN (Electronic)9783642048098
ISBN (Print)9783642048081
DOIs
StatePublished - Jan 1 2010

Fingerprint

screening
Plant Genome
Plastids
Plant Cells
genetically modified organisms
cells
nuclear genome
DNA
Genes
genetic markers
methodology
plastid genome

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

Cite this

Liang, H., Kumar, P. A., Nain, V., Powell, W. A., & Carlson, J. E. (2010). Selection and screening strategies. In Principles and Development (Vol. 1, pp. 85-143). Springer-Verlag Berlin Heidelberg. https://doi.org/10.1007/978-3-642-04809-8_4
Liang, Haiying ; Kumar, P. Ananda ; Nain, Vikrant ; Powell, William A. ; Carlson, John Edward. / Selection and screening strategies. Principles and Development. Vol. 1 Springer-Verlag Berlin Heidelberg, 2010. pp. 85-143
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Liang, H, Kumar, PA, Nain, V, Powell, WA & Carlson, JE 2010, Selection and screening strategies. in Principles and Development. vol. 1, Springer-Verlag Berlin Heidelberg, pp. 85-143. https://doi.org/10.1007/978-3-642-04809-8_4

Selection and screening strategies. / Liang, Haiying; Kumar, P. Ananda; Nain, Vikrant; Powell, William A.; Carlson, John Edward.

Principles and Development. Vol. 1 Springer-Verlag Berlin Heidelberg, 2010. p. 85-143.

Research output: Chapter in Book/Report/Conference proceedingChapter

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Liang H, Kumar PA, Nain V, Powell WA, Carlson JE. Selection and screening strategies. In Principles and Development. Vol. 1. Springer-Verlag Berlin Heidelberg. 2010. p. 85-143 https://doi.org/10.1007/978-3-642-04809-8_4