Abstract
A number of transformation systems have been developed to insert foreign DNA into the appropriate plant genome (nuclear or plastid) (discussed in Chap. 3). However, only a small fraction of the treated cells become transgenic, while the majority of the cells remain untransformed using any of these methods. Thus, effective selection and screening strategies are needed to pick up the rare transgenic lines from a pool of nontransformed cells or plants. To date, more than 50 marker genes and a few molecular techniques have been developed to serve this essential purpose.
| Original language | English (US) |
|---|---|
| Title of host publication | Principles and Development |
| Publisher | Springer-Verlag Berlin Heidelberg |
| Pages | 85-143 |
| Number of pages | 59 |
| Volume | 1 |
| ISBN (Electronic) | 9783642048098 |
| ISBN (Print) | 9783642048081 |
| DOIs | |
| State | Published - Jan 1 2010 |
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All Science Journal Classification (ASJC) codes
- Agricultural and Biological Sciences(all)
Cite this
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Selection and screening strategies. / Liang, Haiying; Kumar, P. Ananda; Nain, Vikrant; Powell, William A.; Carlson, John Edward.
Principles and Development. Vol. 1 Springer-Verlag Berlin Heidelberg, 2010. p. 85-143.Research output: Chapter in Book/Report/Conference proceeding › Chapter
TY - CHAP
T1 - Selection and screening strategies
AU - Liang, Haiying
AU - Kumar, P. Ananda
AU - Nain, Vikrant
AU - Powell, William A.
AU - Carlson, John Edward
PY - 2010/1/1
Y1 - 2010/1/1
N2 - A number of transformation systems have been developed to insert foreign DNA into the appropriate plant genome (nuclear or plastid) (discussed in Chap. 3). However, only a small fraction of the treated cells become transgenic, while the majority of the cells remain untransformed using any of these methods. Thus, effective selection and screening strategies are needed to pick up the rare transgenic lines from a pool of nontransformed cells or plants. To date, more than 50 marker genes and a few molecular techniques have been developed to serve this essential purpose.
AB - A number of transformation systems have been developed to insert foreign DNA into the appropriate plant genome (nuclear or plastid) (discussed in Chap. 3). However, only a small fraction of the treated cells become transgenic, while the majority of the cells remain untransformed using any of these methods. Thus, effective selection and screening strategies are needed to pick up the rare transgenic lines from a pool of nontransformed cells or plants. To date, more than 50 marker genes and a few molecular techniques have been developed to serve this essential purpose.
UR - http://www.scopus.com/inward/record.url?scp=84913614971&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84913614971&partnerID=8YFLogxK
U2 - 10.1007/978-3-642-04809-8_4
DO - 10.1007/978-3-642-04809-8_4
M3 - Chapter
AN - SCOPUS:84913614971
SN - 9783642048081
VL - 1
SP - 85
EP - 143
BT - Principles and Development
PB - Springer-Verlag Berlin Heidelberg
ER -