Selection and screening strategies

Haiying Liang; P. Ananda Kumar; Vikrant Nain; William A. Powell; John Edward Carlson

doi:10.1007/978-3-642-04809-8_4

Selection and screening strategies

Haiying Liang, P. Ananda Kumar, Vikrant Nain, William A. Powell, John Edward Carlson

Research output: Chapter in Book/Report/Conference proceeding › Chapter

3 Scopus citations

Abstract

A number of transformation systems have been developed to insert foreign DNA into the appropriate plant genome (nuclear or plastid) (discussed in Chap. 3). However, only a small fraction of the treated cells become transgenic, while the majority of the cells remain untransformed using any of these methods. Thus, effective selection and screening strategies are needed to pick up the rare transgenic lines from a pool of nontransformed cells or plants. To date, more than 50 marker genes and a few molecular techniques have been developed to serve this essential purpose.

Original language	English (US)
Title of host publication	Principles and Development
Publisher	Springer-Verlag Berlin Heidelberg
Pages	85-143
Number of pages	59
Volume	1
ISBN (Electronic)	9783642048098
ISBN (Print)	9783642048081
DOIs	https://doi.org/10.1007/978-3-642-04809-8_4
State	Published - Jan 1 2010

All Science Journal Classification (ASJC) codes

Agricultural and Biological Sciences(all)

Access to Document

10.1007/978-3-642-04809-8_4

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abstract = "A number of transformation systems have been developed to insert foreign DNA into the appropriate plant genome (nuclear or plastid) (discussed in Chap. 3). However, only a small fraction of the treated cells become transgenic, while the majority of the cells remain untransformed using any of these methods. Thus, effective selection and screening strategies are needed to pick up the rare transgenic lines from a pool of nontransformed cells or plants. To date, more than 50 marker genes and a few molecular techniques have been developed to serve this essential purpose.",

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AU - Nain, Vikrant

AU - Powell, William A.

AU - Carlson, John Edward

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