Selection and screening strategies

Haiying Liang, P. Ananda Kumar, Vikrant Nain, William A. Powell, John Edward Carlson

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

A number of transformation systems have been developed to insert foreign DNA into the appropriate plant genome (nuclear or plastid) (discussed in Chap. 3). However, only a small fraction of the treated cells become transgenic, while the majority of the cells remain untransformed using any of these methods. Thus, effective selection and screening strategies are needed to pick up the rare transgenic lines from a pool of nontransformed cells or plants. To date, more than 50 marker genes and a few molecular techniques have been developed to serve this essential purpose.

Original languageEnglish (US)
Title of host publicationPrinciples and Development
PublisherSpringer-Verlag Berlin Heidelberg
Pages85-143
Number of pages59
Volume1
ISBN (Electronic)9783642048098
ISBN (Print)9783642048081
DOIs
StatePublished - Jan 1 2010

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All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

Cite this

Liang, H., Kumar, P. A., Nain, V., Powell, W. A., & Carlson, J. E. (2010). Selection and screening strategies. In Principles and Development (Vol. 1, pp. 85-143). Springer-Verlag Berlin Heidelberg. https://doi.org/10.1007/978-3-642-04809-8_4