Selective and unidirectional membrane redistribution of an H-2 antigen with an antibody-clustered viral antigen

Relationship to mechanisms of cytotoxic T-cell interactions

B. Geiger, K. L. Rosenthal, Jan Klein, R. M. Zinkernagel, S. J. Singer

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We have studied the co-redistributions of vesicular stomatitis virus (VSV) antigen and of individual H-2 antigens on the surfaces of mouse cells, and in parallel we have also used these VSV-infected cells as targets in cytotoxic T-cell killing experiments. Antibody-induced patching and capping of the VSV antigen caused an extensive co-patching and co-capping of the H-2Kb antigen but not of the H-2Db antigen. In reciprocal experiments, the antibody-induced patching of the H-2Kb or H-2Db antigen did not result in a co-patching of the VSV antigen. Radioimmunassays showed that the relative numbers of H-2Kb, H-2Db, and VSV antigens on the surfaces of the cells exhibiting such nonreciprocal co-redistributions were closely similar. Furthermore, the H-2 restricted cytotoxic T-cell lysis of these target cells showed a marked preference for H-2Kb compared to H-2Db compatibility. We propose that the VSV and H-2 antigens are molecularly independent entities in the unperturbed target cell membrane but that the antibody-induced clustering of the VSV antigen causes a selective and unidirectional co-redistribution (which we designate as syn-capping) of H-2Kb with the VSV antigen clusters. It is suggested that such a T-cell-induced syn-capping process involving an antigen and an H-2 molecule on the target cell may play a critical role in the mechanism of cytotoxic T-cell killing.

Original languageEnglish (US)
Pages (from-to)4603-4607
Number of pages5
JournalUnknown Journal
Volume76
Issue number9
DOIs
StatePublished - Jan 1 1979

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H-2 Antigens
Vesicular Stomatitis
Viral Antigens
Cell Communication
Viruses
T-Lymphocytes
Membranes
Antibodies
Antigens
Surface Antigens
Cluster Analysis
Cell Membrane

All Science Journal Classification (ASJC) codes

  • General

Cite this

Geiger, B. ; Rosenthal, K. L. ; Klein, Jan ; Zinkernagel, R. M. ; Singer, S. J. / Selective and unidirectional membrane redistribution of an H-2 antigen with an antibody-clustered viral antigen : Relationship to mechanisms of cytotoxic T-cell interactions. In: Unknown Journal. 1979 ; Vol. 76, No. 9. pp. 4603-4607.
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Selective and unidirectional membrane redistribution of an H-2 antigen with an antibody-clustered viral antigen : Relationship to mechanisms of cytotoxic T-cell interactions. / Geiger, B.; Rosenthal, K. L.; Klein, Jan; Zinkernagel, R. M.; Singer, S. J.

In: Unknown Journal, Vol. 76, No. 9, 01.01.1979, p. 4603-4607.

Research output: Contribution to journalArticle

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