Selective Growth of Transformed Cell Lines by Rat Liver Perfusate

Mary F. Schuler, Leonard S. Jefferson, Justine Gorodecki, Ronald Glaser, Janet Dietz, Allan Lipton

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Perfused rat liver releases growth-promoting activity for viral, spontaneous, and chemically transformed cells. After 5 days of incubation with perfusate, cell lines 3T12-NY (a spontaneous fibroblast transformant), NQ-T-1 (a chemically transformed fibroblast line), W-8 (a chemically transformed epithelial rat liver cell line), and H-50 (an SV40-transformed hamster fibroblast line) all exhibit significant increase in cell growth above controls. Their respective normal counterparts: 3T3 Cl 42, A31-714, K-16, and HEF are not so stimulated. Within another set, the virally transformed mouse fibroblast cell line, SV3T3, exhibits a 27-fold increase in growth; however, 3T3 (mouse fibroblasts), Py3T3 (polyomatransformed 3T3 cells), SV-FI2-101 (a flat revertant line), and SV-Py-3T3 (a doubly transformed line) are nonresponsive. Perfused rat liver also releases survival activity for SV-3T3 cells. The growth-stimulating activity in liver perfusate is selective for transformed cells. It is suggested that the liver may play a role in supporting neoplasia in vivo.

Original languageEnglish (US)
Pages (from-to)1662-1665
Number of pages4
JournalCancer Research
Issue number6
StatePublished - Jul 1 1977

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research


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