Selective method for the isolation of Sporolactobacillus from food and environmental sources

Stephanie Doores, D. C. Westhoff

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A detection procedure was developed in which a modified Lactobacilli MRS medium was used to enrich for Sporolactobacillus from a variety of foods, feed, soil and environmental samples. Each sample was rinsed with 50 ml of a modified Lactobacilli MRS broth containing 1.0% (w/v) α‐methyl glucoside, 0.1% (w/v) potassium sorbate, 0.00224% (w/v) bromocresol green indicator, adjusted to pH 5·5 with acetic acid and incubated at 37°C for 7 d under 5% CO2. Volumes of 2 ml from each sample were heat shocked at 80°C for 5 min and 0.1 ml spread onto plates of Lactobacilli MRS agar (Difco), pH 5·5 and APT agar (BBL), pH 5·5. Plates were incubated for 5 d and suspect colonies were tested for catalase production, benzidine reaction, nitrate reduction, motility and Gram stain reaction. This method was demonstrated to be selective for Sporolactobacillus.

Original languageEnglish (US)
Pages (from-to)273-280
Number of pages8
JournalJournal of Applied Bacteriology
Volume54
Issue number2
DOIs
StatePublished - Jan 1 1983

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

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