Selective modification of rabbit liver fructose bisphosphatase

M. M. Demaine, Stephen Benkovic

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Chemical modification of rabbit liver fructose 1,6-bisphosphatase by 5,5′-dithiobis-(2-nitrobenzoic acid) results in thiolation of four highly reactive sulfhydryl groups and a diminished sensitivity to AMP inhibition but not loss of enzyme activity. Ethoxyformylation of the histidine groups of fructose 1,6-bisphosphatase does not result in a sharp loss of activity until at least 4 or 5 of the 13 residues have reacted. Exhaustive formylation does abolish the enzyme's activity. These four most reactive sulfhydryl groups and the one or two least easily modified histidine moieties (those responsible for activity) can be protected against modification by fructose-1,6-P2 and to a lesser extent by fructose-6-P. The binding of fructose-1,6-P2 to fructose 1,6-bisphosphatase, however, depends on the presence of structural metal ion since EDTA which removes all endogenous Zn2+ from the protein prevents binding of fructose-1, 6-P2 to the enzyme.

Original languageEnglish (US)
Pages (from-to)308-314
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume205
Issue number2
DOIs
StatePublished - Jan 1 1980

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Fructose-Bisphosphatase
Fructose
Liver
Rabbits
Enzyme activity
Histidine
Enzymes
Structural metals
Nitrobenzoates
Enzyme inhibition
Chemical modification
Adenosine Monophosphate
Protein Binding
Edetic Acid
Metal ions
Metals
Ions

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Demaine, M. M. ; Benkovic, Stephen. / Selective modification of rabbit liver fructose bisphosphatase. In: Archives of Biochemistry and Biophysics. 1980 ; Vol. 205, No. 2. pp. 308-314.
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Selective modification of rabbit liver fructose bisphosphatase. / Demaine, M. M.; Benkovic, Stephen.

In: Archives of Biochemistry and Biophysics, Vol. 205, No. 2, 01.01.1980, p. 308-314.

Research output: Contribution to journalArticle

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AU - Benkovic, Stephen

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AB - Chemical modification of rabbit liver fructose 1,6-bisphosphatase by 5,5′-dithiobis-(2-nitrobenzoic acid) results in thiolation of four highly reactive sulfhydryl groups and a diminished sensitivity to AMP inhibition but not loss of enzyme activity. Ethoxyformylation of the histidine groups of fructose 1,6-bisphosphatase does not result in a sharp loss of activity until at least 4 or 5 of the 13 residues have reacted. Exhaustive formylation does abolish the enzyme's activity. These four most reactive sulfhydryl groups and the one or two least easily modified histidine moieties (those responsible for activity) can be protected against modification by fructose-1,6-P2 and to a lesser extent by fructose-6-P. The binding of fructose-1,6-P2 to fructose 1,6-bisphosphatase, however, depends on the presence of structural metal ion since EDTA which removes all endogenous Zn2+ from the protein prevents binding of fructose-1, 6-P2 to the enzyme.

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