Selenite induction of DNA strand breaks and apoptosis in mouse leukemic L1210 cells

Junxuan Lu, Mark Kaeck, Cheng Jiang, Adrian C. Wilson, Henry J. Thompson

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

The effects of selenite on DNA integrity, cell viability, and long-term proliferative potential of mouse leukemic L1210 cells were examined in this study. Selenite treatment resulted in concentration-dependent increases in DNA single-strand breaks and double-strand breaks, as detected by a modified filter elution assay. A time-course experiment showed that DNA single-strand breaks preceded DNA double-strand breaks. Agarose gel electrophoresis of DNA extracted from selenite-treated cells displayed a nucleosomal fragmentation pattern that is characteristics of apoptotic cell death. The involvement of a Ca2+, Mg2+-dependent endonuclease responsible for DNA double-strand fragmentation was implied by the observation that two inhibitors of endonuclease activity, i.e. aurintricarboxylic acid and zinc, blocked selenite-induced DNA double-strand breaks. These inhibitors also prevented selenite-induced cell death as defined by loss of ability to exclude trypan blue dye. Selenite treatment severely impaired the colony-forming ability of cell capable of trypan blue exclusion. The induction of DNA strand breaks and commitment to apoptosis may explain the selenite-mediated growth inhibition and loss of long-term proliferative potential.

Original languageEnglish (US)
Pages (from-to)1531-1535
Number of pages5
JournalBiochemical Pharmacology
Volume47
Issue number9
DOIs
StatePublished - Apr 29 1994

Fingerprint

Selenious Acid
DNA Breaks
Apoptosis
DNA
Single-Stranded DNA Breaks
Trypan Blue
Double-Stranded DNA Breaks
Cell death
Cell Death
Aurintricarboxylic Acid
Cells
Agar Gel Electrophoresis
Endonucleases
Deoxyribonuclease I
Electrophoresis
Cell Survival
Sepharose
Coloring Agents
Zinc
Assays

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Pharmacology

Cite this

Lu, Junxuan ; Kaeck, Mark ; Jiang, Cheng ; Wilson, Adrian C. ; Thompson, Henry J. / Selenite induction of DNA strand breaks and apoptosis in mouse leukemic L1210 cells. In: Biochemical Pharmacology. 1994 ; Vol. 47, No. 9. pp. 1531-1535.
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Selenite induction of DNA strand breaks and apoptosis in mouse leukemic L1210 cells. / Lu, Junxuan; Kaeck, Mark; Jiang, Cheng; Wilson, Adrian C.; Thompson, Henry J.

In: Biochemical Pharmacology, Vol. 47, No. 9, 29.04.1994, p. 1531-1535.

Research output: Contribution to journalArticle

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