TY - JOUR
T1 - Selenium reduces enterohemorrhagic Escherichia coli O157:H7 verotoxin production and globotriaosylceramide receptor expression on host cells
AU - Surendran-Nair, Meera
AU - Kollanoor-Johny, Anup
AU - Ananda-Baskaran, Sangeetha
AU - Norris, Carol
AU - Lee, Ji Young
AU - Venkitanarayanan, Kumar
N1 - Publisher Copyright:
© 2016 Future Medicine Ltd.
PY - 2016/6
Y1 - 2016/6
N2 - Aim: This study investigated the efficacy of selenium (Se) in reducing Escherichia coli O157:H7 verotoxin production and toxin gene expression. Additionally, the effect of Se on globotriaosylceramide (Gb3) receptor in human lymphoma cells was determined. Materials & methods: The effect of Se on verotoxin synthesis was determined by standard ELISA, whereas its effect on Gb3 receptor was determined by flow cytometry and real-time quantitative PCR. Results & conclusions: Se reduced extracellular and intracellular verotoxin concentration by 40-60% and 80-90%, respectively (p < 0.05), and downregulated verotoxin genes (p < 0.05). Se reduced Gb3 receptor synthesis in lymphoma cells, and real-time quantitative PCR data revealed a significant downregulation of LacCer synthase gene (GalT2) involved in Gb3 synthesis. Further studies are warranted to validate these results in an appropriate animal model.
AB - Aim: This study investigated the efficacy of selenium (Se) in reducing Escherichia coli O157:H7 verotoxin production and toxin gene expression. Additionally, the effect of Se on globotriaosylceramide (Gb3) receptor in human lymphoma cells was determined. Materials & methods: The effect of Se on verotoxin synthesis was determined by standard ELISA, whereas its effect on Gb3 receptor was determined by flow cytometry and real-time quantitative PCR. Results & conclusions: Se reduced extracellular and intracellular verotoxin concentration by 40-60% and 80-90%, respectively (p < 0.05), and downregulated verotoxin genes (p < 0.05). Se reduced Gb3 receptor synthesis in lymphoma cells, and real-time quantitative PCR data revealed a significant downregulation of LacCer synthase gene (GalT2) involved in Gb3 synthesis. Further studies are warranted to validate these results in an appropriate animal model.
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U2 - 10.2217/fmb.16.16
DO - 10.2217/fmb.16.16
M3 - Article
C2 - 27191971
AN - SCOPUS:84971537233
VL - 11
SP - 745
EP - 756
JO - Future Microbiology
JF - Future Microbiology
SN - 1746-0913
IS - 6
ER -