Self-incompatibility in Petunia inflata: isolation and characterization of cDNAs encoding three S-allele-associated proteins

Yunjun Ai, Anuradha Singh, Craig E. Coleman, Thomas R. Ioerger, Ahmed Kheyr-Pour, Teh-hui Kao

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

We have identified three alleles of the S-locus controlling self-incompatibility and their associated pistil proteins in Petunia inflata, a species that displays monofactorial gametophytic self-incompatibility. These S-allele-associated proteins (S-proteins) are pistil specific, and their levels are developmentally regulated. The amino-terminal sequences determined for the three S-proteins are highly conserved and show considerable homology to those of S-proteins from Petunia hybrida, Nicotiana alata and Lycopersicon peruvianum, three other species of the Solanaceae that also exhibit gametophytic self-incompatibility. cDNA clones encoding the three S-proteins were isolated and sequenced. Comparison of their deduced amino acid sequences reveals an average homology of 75.6%, with conserved and variable residue interspersed throughout the protein. Of the 137 conserved residues, 53 are also conserved in the N. alata S-proteins studies so far; of the 64 variable residues, 29 were identified as hypervariable based on calculation of the Similarity Index. There is only one hypervariable region of significant length, and it consists of eight consecutive hypervariable residues. This region correspond approximately to the hypervariable region HV2 identified in N. alata S-proteins. Of the two classes of N. alata S-proteins previously identified, one class exhibits greater homology to the three P. inflata S-proteins reported here than to the other class of N. alata S-proteins.

Original languageEnglish (US)
Pages (from-to)130-138
Number of pages9
JournalSexual Plant Reproduction
Volume3
Issue number2
DOIs
StatePublished - May 1 1990

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Petunia integrifolia
Petunia
Complementary DNA
Alleles
alleles
Nicotiana alata
Proteins
proteins
pistil
Solanaceae
Solanum peruvianum
Petunia hybrida
Tobacco
Amino Acid Sequence

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Ai, Yunjun ; Singh, Anuradha ; Coleman, Craig E. ; Ioerger, Thomas R. ; Kheyr-Pour, Ahmed ; Kao, Teh-hui. / Self-incompatibility in Petunia inflata : isolation and characterization of cDNAs encoding three S-allele-associated proteins. In: Sexual Plant Reproduction. 1990 ; Vol. 3, No. 2. pp. 130-138.
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Self-incompatibility in Petunia inflata : isolation and characterization of cDNAs encoding three S-allele-associated proteins. / Ai, Yunjun; Singh, Anuradha; Coleman, Craig E.; Ioerger, Thomas R.; Kheyr-Pour, Ahmed; Kao, Teh-hui.

In: Sexual Plant Reproduction, Vol. 3, No. 2, 01.05.1990, p. 130-138.

Research output: Contribution to journalArticle

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AU - Ai, Yunjun

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