Self-sacrifice in radical S-adenosylmethionine proteins

Squire J. Booker, Robert M. Cicchillo, Tyler L. Grove

Research output: Contribution to journalReview article

88 Scopus citations

Abstract

The radical SAM superfamily of metalloproteins catalyze the reductive cleavage of S-adenosyl-l-methionine to generate a 5′-deoxyadenosyl radical (5′-dA{radical dot}) intermediate that is obligate for turnover. The 5′-dA{radical dot} acts as a potent oxidant, initiating turnover by abstracting a hydrogen atom from an appropriate substrate. A special class of these enzymes use this strategy to functionalize unactivated C-H bonds by insertion of sulfur atoms. This review will describe the characterization of three members of this class - biotin synthase, lipoyl synthase, and MiaB protein - each of which has been shown to cannibalize itself during turnover.

Original languageEnglish (US)
Pages (from-to)543-552
Number of pages10
JournalCurrent Opinion in Chemical Biology
Volume11
Issue number5
DOIs
StatePublished - Oct 1 2007

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this