Separation of fatty acid esters of retinol by high-performance liquid chromatography

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Abstract

Long-chain fatty acid esters of retinol comprise the major storage form of vitamin A in numerous tissues. It has long been recognized that various organs have characteristic patterns of esterified retinol and that differences may exist among animal species in the retinyl ester composition within a particular organ such as the liver. Thin-layer chromatographic methods have now been replaced by high-performance liquid chromatography (HPLC) procedures that permit excellent resolution, as well as quantification, of individual fatty acid esters of retinol. This chapter presents a brief review and comparison of four procedures that were developed for the individual analysis of retinyl ester species. All are based on similar principles and provide rather comparable separations, but they utilize different column and solvent combinations.

Original languageEnglish (US)
Pages (from-to)81-84
Number of pages4
JournalMethods in enzymology
Volume189
Issue numberC
DOIs
StatePublished - Jan 1 1990

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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