Separation of isomeric lysophospholipids by reverse phase HPLC

Michael H. Creer, Richard W. Gross

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A reverse-phase high performance liquid chromatography (HPLC) method was developed which resolved isomers of lysophosphatidylcholine (LPC) differing in the location of the aliphatic chain (sn-1 or sn-2 position) and the position (Δ6 or Δ9) or geometric configuration (cis or trans) of the olefin group in monounsaturated species. LPC isomers containing an acyl substituent at the sn-2 position eluted before their 1-acyl-sn-glycero-3-phosphocholine (1-acyl LPC) counterparts. The retention times of both the sn-1 and sn-2 isomers of monounsaturated species increased in the order Δ9-cis < Δ9-trans < Δ6-cis. The integrated ultraviolet absorbance (203 nm) in binary mixtures of the Δ9-cis and Δ6-cis 2-acyl lysophospholipid isomers correlated with the lipid phosphorus content of corresponding column eluates (r-0.994). Thus, the present method will facilitate synthesis of isomerically pure diradylphospholipids by providing homogeneous lysophospholipid precursors and help simplify the quantitative analysis of unsaturated lysophospholipid species.

Original languageEnglish (US)
Pages (from-to)922-928
Number of pages7
JournalLipids
Volume20
Issue number12
DOIs
StatePublished - Dec 1 1985

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Lysophospholipids
High performance liquid chromatography
Reverse-Phase Chromatography
Isomers
Lysophosphatidylcholines
High Pressure Liquid Chromatography
Phosphorylcholine
Alkenes
Phosphorus
Binary mixtures
Lipids
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

Cite this

Creer, Michael H. ; Gross, Richard W. / Separation of isomeric lysophospholipids by reverse phase HPLC. In: Lipids. 1985 ; Vol. 20, No. 12. pp. 922-928.
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Separation of isomeric lysophospholipids by reverse phase HPLC. / Creer, Michael H.; Gross, Richard W.

In: Lipids, Vol. 20, No. 12, 01.12.1985, p. 922-928.

Research output: Contribution to journalArticle

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AB - A reverse-phase high performance liquid chromatography (HPLC) method was developed which resolved isomers of lysophosphatidylcholine (LPC) differing in the location of the aliphatic chain (sn-1 or sn-2 position) and the position (Δ6 or Δ9) or geometric configuration (cis or trans) of the olefin group in monounsaturated species. LPC isomers containing an acyl substituent at the sn-2 position eluted before their 1-acyl-sn-glycero-3-phosphocholine (1-acyl LPC) counterparts. The retention times of both the sn-1 and sn-2 isomers of monounsaturated species increased in the order Δ9-cis < Δ9-trans < Δ6-cis. The integrated ultraviolet absorbance (203 nm) in binary mixtures of the Δ9-cis and Δ6-cis 2-acyl lysophospholipid isomers correlated with the lipid phosphorus content of corresponding column eluates (r-0.994). Thus, the present method will facilitate synthesis of isomerically pure diradylphospholipids by providing homogeneous lysophospholipid precursors and help simplify the quantitative analysis of unsaturated lysophospholipid species.

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