Separation of Two Forms of Anthranilate Synthetase from 5‐Methyltryptophar Susceptible and ‐Resistant Cultured Solarium tuberosumCells

John Edward Carlson, J. M. WIDHOLM

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Potato cell suspension cultures (Solanum tuberosumL. cv. Merrimack) have been selected which are resistant to growth inhibition by D,L‐5‐methyltryptophan. Anthranilate synthetase activity in crude extracts from resistant cells was less sensitive to feedback inhibition by L‐tryptophan and D,L‐5‐methyltryptophan than the activity from the sensitive line. This altered feedback control apparently accounts for the cell's resistance to growth inhibition since there is a 48‐fold increase in free tryptophan in one of the resistant cell lines. Preparative polyacrylamide gel electro‐phoresis separated feedback‐sensitive and ‐resistant forms of anthranilate synthetase in extracts from both 5‐methyltryptophan‐susceptible and ‐resistant cells, with a predominance of the corresponding form in the respective cell type. The anthranilate synthetase activity from the 5‐methyltryptophan‐resistant line was inactivated more slowly by incubation of crude extracts at 50°C than the activity from the sensitive line. These results suggest the presence of two isoenzymes of anthranilate synthetase in cultured potato cells.

Original languageEnglish (US)
Pages (from-to)251-255
Number of pages5
JournalPhysiologia Plantarum
Volume44
Issue number3
DOIs
StatePublished - Jan 1 1978

Fingerprint

Anthranilate Synthase
anthranilate synthase
Solanum tuberosum
Complex Mixtures
growth retardation
cells
extracts
Solanum
potatoes
Growth
Tryptophan
Isoenzymes
Polyacrylamide Gel Electrophoresis
Cultured Cells
Suspensions
Cell Culture Techniques
tryptophan
cell suspension culture
polyacrylamide gel electrophoresis
isozymes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

Cite this

@article{481aabdced5f4b4f9d2ad4cf5b4ee7e2,
title = "Separation of Two Forms of Anthranilate Synthetase from 5‐Methyltryptophar Susceptible and ‐Resistant Cultured Solarium tuberosumCells",
abstract = "Potato cell suspension cultures (Solanum tuberosumL. cv. Merrimack) have been selected which are resistant to growth inhibition by D,L‐5‐methyltryptophan. Anthranilate synthetase activity in crude extracts from resistant cells was less sensitive to feedback inhibition by L‐tryptophan and D,L‐5‐methyltryptophan than the activity from the sensitive line. This altered feedback control apparently accounts for the cell's resistance to growth inhibition since there is a 48‐fold increase in free tryptophan in one of the resistant cell lines. Preparative polyacrylamide gel electro‐phoresis separated feedback‐sensitive and ‐resistant forms of anthranilate synthetase in extracts from both 5‐methyltryptophan‐susceptible and ‐resistant cells, with a predominance of the corresponding form in the respective cell type. The anthranilate synthetase activity from the 5‐methyltryptophan‐resistant line was inactivated more slowly by incubation of crude extracts at 50°C than the activity from the sensitive line. These results suggest the presence of two isoenzymes of anthranilate synthetase in cultured potato cells.",
author = "Carlson, {John Edward} and WIDHOLM, {J. M.}",
year = "1978",
month = "1",
day = "1",
doi = "10.1111/j.1399-3054.1978.tb08627.x",
language = "English (US)",
volume = "44",
pages = "251--255",
journal = "Physiologia Plantarum",
issn = "0031-9317",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Separation of Two Forms of Anthranilate Synthetase from 5‐Methyltryptophar Susceptible and ‐Resistant Cultured Solarium tuberosumCells

AU - Carlson, John Edward

AU - WIDHOLM, J. M.

PY - 1978/1/1

Y1 - 1978/1/1

N2 - Potato cell suspension cultures (Solanum tuberosumL. cv. Merrimack) have been selected which are resistant to growth inhibition by D,L‐5‐methyltryptophan. Anthranilate synthetase activity in crude extracts from resistant cells was less sensitive to feedback inhibition by L‐tryptophan and D,L‐5‐methyltryptophan than the activity from the sensitive line. This altered feedback control apparently accounts for the cell's resistance to growth inhibition since there is a 48‐fold increase in free tryptophan in one of the resistant cell lines. Preparative polyacrylamide gel electro‐phoresis separated feedback‐sensitive and ‐resistant forms of anthranilate synthetase in extracts from both 5‐methyltryptophan‐susceptible and ‐resistant cells, with a predominance of the corresponding form in the respective cell type. The anthranilate synthetase activity from the 5‐methyltryptophan‐resistant line was inactivated more slowly by incubation of crude extracts at 50°C than the activity from the sensitive line. These results suggest the presence of two isoenzymes of anthranilate synthetase in cultured potato cells.

AB - Potato cell suspension cultures (Solanum tuberosumL. cv. Merrimack) have been selected which are resistant to growth inhibition by D,L‐5‐methyltryptophan. Anthranilate synthetase activity in crude extracts from resistant cells was less sensitive to feedback inhibition by L‐tryptophan and D,L‐5‐methyltryptophan than the activity from the sensitive line. This altered feedback control apparently accounts for the cell's resistance to growth inhibition since there is a 48‐fold increase in free tryptophan in one of the resistant cell lines. Preparative polyacrylamide gel electro‐phoresis separated feedback‐sensitive and ‐resistant forms of anthranilate synthetase in extracts from both 5‐methyltryptophan‐susceptible and ‐resistant cells, with a predominance of the corresponding form in the respective cell type. The anthranilate synthetase activity from the 5‐methyltryptophan‐resistant line was inactivated more slowly by incubation of crude extracts at 50°C than the activity from the sensitive line. These results suggest the presence of two isoenzymes of anthranilate synthetase in cultured potato cells.

UR - http://www.scopus.com/inward/record.url?scp=84981633398&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84981633398&partnerID=8YFLogxK

U2 - 10.1111/j.1399-3054.1978.tb08627.x

DO - 10.1111/j.1399-3054.1978.tb08627.x

M3 - Article

AN - SCOPUS:84981633398

VL - 44

SP - 251

EP - 255

JO - Physiologia Plantarum

JF - Physiologia Plantarum

SN - 0031-9317

IS - 3

ER -