Sequence analysis of linked maize 22 kDa α-zein genes

Gary Allen Thompson, David R. Siemieniak, Leang C. Sieu, Jerry L. Slightom, Brian A. Larkins

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We have determined the nucleotide sequence of a 7343 bp zein genomic clone (gZ22.8H3) from the maize inbred W64A. Computer-aided analysis of the DNA sequence revealed two contiguous 22 kDa α-zein genes. The 5′ gene (gZ22.8) encodes a complete polypeptide and contains putative regulatory sequences in both the 5′ and 3′ flanking regions that are typical of zein genes. In contrast, the 3′ gene (ψgZ22.8) appears to be a pseudogene, because it contains numerous insertions and deletions that would prevent translation of the mRNA. Alignment of the 5′ and 3′ flanking sequences of both genes indicated that they resulted from a 3.3 kb DNA duplication event.

Original languageEnglish (US)
Pages (from-to)827-833
Number of pages7
JournalPlant Molecular Biology
Volume18
Issue number4
DOIs
StatePublished - Feb 1 1992

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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    Thompson, G. A., Siemieniak, D. R., Sieu, L. C., Slightom, J. L., & Larkins, B. A. (1992). Sequence analysis of linked maize 22 kDa α-zein genes. Plant Molecular Biology, 18(4), 827-833. https://doi.org/10.1007/BF00020030