Sequence diversity of pistil S-proteins associated with gametophytic self-incompatibility in Nicotiana alata

Ahmed Kheyr-Pour, Scott B. Bintrim, Thomas R. Ioerger, Rene Remy, Scott A. Hammond, Teh-hui Kao

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

In order to study the extent and nature of differences among various S-allele-associated proteins in N. alata, we carried out comparative studies of seven such proteins. We first isolated and sequenced cDNA clones for the Sz-, SF11-, S1-, and Sa-alleles, and then we compared the deduced amino acid sequences both of these four S-proteins and of three previously published S2-, S3-, and S6-proteins. This comparison revealed (1) an average homology of 53.8% among the seven proteins and (2) two homology classes, with Sz and SF11 in one class and S1, S2, S3, and S6 in the other class. There are 60 conserved residues, including 9 cysteines. Of the 144 variable residues, 50 were identified as hypervariable based on a calculation of their Similarity Indices. Although conserved, variable, and hypervariable residues are dispersed throughout the protein, some are clustered to form five conserved, five hypervariable, and a number of variable regions. Those variable sites which contain residues conserved within one class of S-proteins but different between classes might provide a clue to the evolutionary relationship of these two classes of S-proteins. The hypervariable residues, which account for sequence variability, may contribute to allelic specificity.

Original languageEnglish (US)
Pages (from-to)88-97
Number of pages10
JournalSexual Plant Reproduction
Volume3
Issue number2
DOIs
StatePublished - May 1 1990

Fingerprint

Nicotiana alata
Protein S
pistil
Tobacco
S 6
Proteins
proteins
Alleles
Cysteine
Amino Acid Sequence
alleles
Complementary DNA
Clone Cells
sequence diversity
cysteine
amino acid sequences
clones

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Kheyr-Pour, Ahmed ; Bintrim, Scott B. ; Ioerger, Thomas R. ; Remy, Rene ; Hammond, Scott A. ; Kao, Teh-hui. / Sequence diversity of pistil S-proteins associated with gametophytic self-incompatibility in Nicotiana alata. In: Sexual Plant Reproduction. 1990 ; Vol. 3, No. 2. pp. 88-97.
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Sequence diversity of pistil S-proteins associated with gametophytic self-incompatibility in Nicotiana alata. / Kheyr-Pour, Ahmed; Bintrim, Scott B.; Ioerger, Thomas R.; Remy, Rene; Hammond, Scott A.; Kao, Teh-hui.

In: Sexual Plant Reproduction, Vol. 3, No. 2, 01.05.1990, p. 88-97.

Research output: Contribution to journalArticle

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