Sequencing, distribution, and inactivation of the dipeptidase A gene (pepDA) from Lactobacillus helveticus CNRZ32

Edward G. Dudley, Ann C. Husgen, Wen He, James L. Steele

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Previously, the gene for a general dipeptidase (pepDA) was isolated from a gene bank of Lactobacillus helveticus CNRZ32. The pepDA gene consists of a 1,422-bp open reading frame which could encode a polypeptide of 53.5 kDa. No significant identity was found between the deduced amino acid sequence of the pepDA product and the sequence for other polypeptides reported in GenBank. Southern hybridization studies with a pepDA probe indicated that the nucleotide sequence for pepDA is not well conserved among a variety of lactic acid bacteria. Growth studies indicated that a pepDA deletion had no detectable effect on growth rate or acid production by L. helveticus CNRZ32 in milk. Furthermore, no difference in total cellular dipeptidase activity was detected between the mutant and wild-type strains during logarithmic growth in MRS medium.

Original languageEnglish (US)
Pages (from-to)701-704
Number of pages4
JournalJournal of bacteriology
Volume178
Issue number3
DOIs
StatePublished - Feb 1996

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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