Sequential recruitment of SAGA and TFIID in a genomic response to DNA damage in Saccharomyces cerevisiae

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Eukaryotic genes respond to their environment by changing the expression of selected genes. The question we address here is whether distinct transcriptional responses to different environmental signals elicit distinct modes of assembly of the transcription machinery. In particular, we examine transcription complex assembly by the stress-directed SAGA complex versus the housekeeping assembly factor TFIID. We focus on genomic responses to the DNA damaging agent methyl methanesulfonate (MMS) in comparison to responses to acute heat shock, looking at changes in genome-wide factor occupancy measured by chromatin immunoprecipitation-microchip (ChIP-chip) and ChIP-sequencing analyses. Our data suggest that MMS-induced genes undergo transcription complex assembly sequentially, first involving SAGA and then involving a slower TFIID recruitment, whereas heat shock genes utilize the SAGA and TFIID pathways rapidly and in parallel. Also Crt1, the repressor of model MMS-inducible ribonucleotide reductase genes, was found not to play a wider role in repression of DNA damage-inducible genes. Taken together, our findings reveal a distinct involvement of gene and chromatin regulatory factors in response to DNA damage versus heat shock and suggest different implementations of the SAGA and TFIID assembly pathways that may depend upon whether a sustained or transient change in gene expression ensues.

Original languageEnglish (US)
Pages (from-to)190-202
Number of pages13
JournalMolecular and cellular biology
Volume31
Issue number1
DOIs
StatePublished - Jan 1 2011

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Transcription Factor TFIID
DNA Damage
Saccharomyces cerevisiae
Methyl Methanesulfonate
Shock
Hot Temperature
Genes
Ribonucleotide Reductases
Gene Expression
Housekeeping
Chromatin Immunoprecipitation
Regulator Genes
Chromatin
Genome
DNA

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Molecular Biology

Cite this

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Sequential recruitment of SAGA and TFIID in a genomic response to DNA damage in Saccharomyces cerevisiae. / Ghosh, Sujana; Pugh, Benjamin Franklin.

In: Molecular and cellular biology, Vol. 31, No. 1, 01.01.2011, p. 190-202.

Research output: Contribution to journalArticle

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