Sequestration and metabolism of host cell arginine by the intraerythrocytic malaria parasite Plasmodium falciparum

Simon A. Cobbold, Manuel Llinas, Kiaran Kirk

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Human erythrocytes have an active nitric oxide synthase, which converts arginine into citrulline and nitric oxide (NO). NO serves several important functions, including the maintenance of normal erythrocyte deformability, thereby ensuring efficient passage of the red blood cell through narrow microcapillaries. Here, we show that following invasion by the malaria parasite Plasmodium falciparum the arginine pool in the host erythrocyte compartment is sequestered and metabolized by the parasite. Arginine from the extracellular medium enters the infected cell via endogenous host cell transporters and is taken up by the intracellular parasite by a high-affinity cationic amino acid transporter at the parasite surface. Within the parasite arginine is metabolized into citrulline and ornithine. The uptake and metabolism of arginine by the parasite deprive the erythrocyte of the substrate required for NO production and may contribute to the decreased deformability of infected erythrocytes.

Original languageEnglish (US)
Article numberA820
Pages (from-to)820-830
Number of pages11
JournalCellular Microbiology
Volume18
Issue number6
DOIs
StatePublished - Jun 1 2016

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Falciparum Malaria
Arginine
Parasites
Erythrocytes
Erythrocyte Deformability
Citrulline
Nitric Oxide
Basic Amino Acid Transport Systems
Ornithine
Nitric Oxide Synthase
Maintenance

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

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abstract = "Human erythrocytes have an active nitric oxide synthase, which converts arginine into citrulline and nitric oxide (NO). NO serves several important functions, including the maintenance of normal erythrocyte deformability, thereby ensuring efficient passage of the red blood cell through narrow microcapillaries. Here, we show that following invasion by the malaria parasite Plasmodium falciparum the arginine pool in the host erythrocyte compartment is sequestered and metabolized by the parasite. Arginine from the extracellular medium enters the infected cell via endogenous host cell transporters and is taken up by the intracellular parasite by a high-affinity cationic amino acid transporter at the parasite surface. Within the parasite arginine is metabolized into citrulline and ornithine. The uptake and metabolism of arginine by the parasite deprive the erythrocyte of the substrate required for NO production and may contribute to the decreased deformability of infected erythrocytes.",
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Sequestration and metabolism of host cell arginine by the intraerythrocytic malaria parasite Plasmodium falciparum. / Cobbold, Simon A.; Llinas, Manuel; Kirk, Kiaran.

In: Cellular Microbiology, Vol. 18, No. 6, A820, 01.06.2016, p. 820-830.

Research output: Contribution to journalArticle

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