Serum dioxin concentrations and quality of ovarian function in women of Seveso

Marcella Warner, Brenda Eskenazi, David L. Olive, Stevens Samuels, Sunita Quick-Miles, Paolo Vercellini, Pier Mario Gerthoux, Larry Needham, Donald George Jr Patterson, Paolo Mocarelli

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Abstract

Background: Although 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) has been associated with alterations in ovarian function and hormones in animals, it has not been studied in humans. On 10 July 1976, an explosion exposed residents of Seveso, Italy, to the highest levels of TCDD in a population. Twenty years later, we initiated the Seveso Women's Health Study to study reproductive health. Objective: We related TCDD levels measured in sera collected near the time of explosion and ovarian function (ovarian cysts, ovarian follicles, ovulation rate, serum hormones) at follow-up. Methods: We included 363 women who were 20-40 years of age and nonusers of oral contraceptives. We examined the relationship of 1976 serum TCDD levels with ultrasound-detected ovarian follicles among 96 women in the menstrual follicular phase and serum hormone levels (estradiol, progesterone) among 129 women in the menstrual luteal phase at follow-up. Ovulation was defined by serum progesterone levels > 3 ng/mL. Results: The median serum TCDD level was 77.3 ppt, lipid-adjusted. Serum TCDD was not associated with number or size of ovarian follicles. Of women in the luteal phase, 87 (67%) ovulated. Serum log 10TCDD was not associated with odds of ovulation [adjusted odds ratio = 0.99; 95% confidence interval (CI), 0.5 to 1.9]. Among those who had ovulated, serum log 10TCDD was not associated with serum progesterone [adjusted beta (adj-β) = -0.70; 95% CI, -2.4 to 1.0] or estradiol (adj-β = -1.81; 95% CI, -10.4 to 6.8). Conclusions: We found no clear evidence that 1976 TCDD exposure was associated with ovarian function 20 years later in women exposed to relatively high levels in Seveso, Italy.

Original languageEnglish (US)
Pages (from-to)336-340
Number of pages5
JournalEnvironmental Health Perspectives
Volume115
Issue number3
DOIs
StatePublished - Mar 1 2007

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Dioxins
dioxin
serum
Serum
Progesterone
Ovarian Follicle
Ovulation
Hormones
confidence interval
hormone
Explosions
Estradiol
Luteal Phase
Confidence Intervals
Italy
explosion
Oral Contraceptives
woman
1,4-dioxin
womens health

All Science Journal Classification (ASJC) codes

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health

Cite this

Warner, M., Eskenazi, B., Olive, D. L., Samuels, S., Quick-Miles, S., Vercellini, P., ... Mocarelli, P. (2007). Serum dioxin concentrations and quality of ovarian function in women of Seveso. Environmental Health Perspectives, 115(3), 336-340. https://doi.org/10.1289/ehp.9667
Warner, Marcella ; Eskenazi, Brenda ; Olive, David L. ; Samuels, Stevens ; Quick-Miles, Sunita ; Vercellini, Paolo ; Gerthoux, Pier Mario ; Needham, Larry ; Patterson, Donald George Jr ; Mocarelli, Paolo. / Serum dioxin concentrations and quality of ovarian function in women of Seveso. In: Environmental Health Perspectives. 2007 ; Vol. 115, No. 3. pp. 336-340.
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abstract = "Background: Although 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) has been associated with alterations in ovarian function and hormones in animals, it has not been studied in humans. On 10 July 1976, an explosion exposed residents of Seveso, Italy, to the highest levels of TCDD in a population. Twenty years later, we initiated the Seveso Women's Health Study to study reproductive health. Objective: We related TCDD levels measured in sera collected near the time of explosion and ovarian function (ovarian cysts, ovarian follicles, ovulation rate, serum hormones) at follow-up. Methods: We included 363 women who were 20-40 years of age and nonusers of oral contraceptives. We examined the relationship of 1976 serum TCDD levels with ultrasound-detected ovarian follicles among 96 women in the menstrual follicular phase and serum hormone levels (estradiol, progesterone) among 129 women in the menstrual luteal phase at follow-up. Ovulation was defined by serum progesterone levels > 3 ng/mL. Results: The median serum TCDD level was 77.3 ppt, lipid-adjusted. Serum TCDD was not associated with number or size of ovarian follicles. Of women in the luteal phase, 87 (67{\%}) ovulated. Serum log 10TCDD was not associated with odds of ovulation [adjusted odds ratio = 0.99; 95{\%} confidence interval (CI), 0.5 to 1.9]. Among those who had ovulated, serum log 10TCDD was not associated with serum progesterone [adjusted beta (adj-β) = -0.70; 95{\%} CI, -2.4 to 1.0] or estradiol (adj-β = -1.81; 95{\%} CI, -10.4 to 6.8). Conclusions: We found no clear evidence that 1976 TCDD exposure was associated with ovarian function 20 years later in women exposed to relatively high levels in Seveso, Italy.",
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Warner, M, Eskenazi, B, Olive, DL, Samuels, S, Quick-Miles, S, Vercellini, P, Gerthoux, PM, Needham, L, Patterson, DGJ & Mocarelli, P 2007, 'Serum dioxin concentrations and quality of ovarian function in women of Seveso', Environmental Health Perspectives, vol. 115, no. 3, pp. 336-340. https://doi.org/10.1289/ehp.9667

Serum dioxin concentrations and quality of ovarian function in women of Seveso. / Warner, Marcella; Eskenazi, Brenda; Olive, David L.; Samuels, Stevens; Quick-Miles, Sunita; Vercellini, Paolo; Gerthoux, Pier Mario; Needham, Larry; Patterson, Donald George Jr; Mocarelli, Paolo.

In: Environmental Health Perspectives, Vol. 115, No. 3, 01.03.2007, p. 336-340.

Research output: Contribution to journalArticle

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T1 - Serum dioxin concentrations and quality of ovarian function in women of Seveso

AU - Warner, Marcella

AU - Eskenazi, Brenda

AU - Olive, David L.

AU - Samuels, Stevens

AU - Quick-Miles, Sunita

AU - Vercellini, Paolo

AU - Gerthoux, Pier Mario

AU - Needham, Larry

AU - Patterson, Donald George Jr

AU - Mocarelli, Paolo

PY - 2007/3/1

Y1 - 2007/3/1

N2 - Background: Although 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) has been associated with alterations in ovarian function and hormones in animals, it has not been studied in humans. On 10 July 1976, an explosion exposed residents of Seveso, Italy, to the highest levels of TCDD in a population. Twenty years later, we initiated the Seveso Women's Health Study to study reproductive health. Objective: We related TCDD levels measured in sera collected near the time of explosion and ovarian function (ovarian cysts, ovarian follicles, ovulation rate, serum hormones) at follow-up. Methods: We included 363 women who were 20-40 years of age and nonusers of oral contraceptives. We examined the relationship of 1976 serum TCDD levels with ultrasound-detected ovarian follicles among 96 women in the menstrual follicular phase and serum hormone levels (estradiol, progesterone) among 129 women in the menstrual luteal phase at follow-up. Ovulation was defined by serum progesterone levels > 3 ng/mL. Results: The median serum TCDD level was 77.3 ppt, lipid-adjusted. Serum TCDD was not associated with number or size of ovarian follicles. Of women in the luteal phase, 87 (67%) ovulated. Serum log 10TCDD was not associated with odds of ovulation [adjusted odds ratio = 0.99; 95% confidence interval (CI), 0.5 to 1.9]. Among those who had ovulated, serum log 10TCDD was not associated with serum progesterone [adjusted beta (adj-β) = -0.70; 95% CI, -2.4 to 1.0] or estradiol (adj-β = -1.81; 95% CI, -10.4 to 6.8). Conclusions: We found no clear evidence that 1976 TCDD exposure was associated with ovarian function 20 years later in women exposed to relatively high levels in Seveso, Italy.

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Warner M, Eskenazi B, Olive DL, Samuels S, Quick-Miles S, Vercellini P et al. Serum dioxin concentrations and quality of ovarian function in women of Seveso. Environmental Health Perspectives. 2007 Mar 1;115(3):336-340. https://doi.org/10.1289/ehp.9667