Serum homocysteine: Interplay with other circulating and genetic factors in association to Alzheimer's type dementia

José F. Cascalheira, Sara S. João, Sandra S. Pinhanços, Maria Rita Castro, Manuela Palmeira, Sofia Almeida, Maria C. Faria, Fernanda C. Domingues

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Objectives: To study the interplay between serum concentrations of homocysteine, steroid hormones and vitamins B and mutations in 5,10-methylenetetrahydrofolate reductase (MTHFR) gene, in association to Alzheimer's type dementia (ATD). Design and methods: Case-control study including 19 individuals diagnosed with ATD and 36 healthy controls. Serum concentrations of the analytes were determined and MTHFR 1298A → C mutation was screened by PCR-RFLP. Results: Multivariable logistic regression analysis identified homocysteine (OR = 1.92, P < 0.01), cholesterol (OR = 1.14, P < 0.001), estradiol (OR = 0.728, P < 0.001), uric acid (OR = 2.42, P < 0.02), vitamin B12 (OR = 0.984, P < 0.004) and MTHFR 1298A → C mutation (OR = 6.01, P < 0.04) as independent predictors of ATD. Positive interaction between homocysteine and uric acid, creatinine, urea or cortisol (P < 0.02) and negative interaction between homocysteine and vitamin B12 or MTHFR 1298A → C mutation (P < 0.03) were observed. Conclusions: High serum concentrations of homocysteine, cholesterol and uric acid, and low concentrations of estradiol and vitamin B12, as well as the MTHFR 1298A → C mutation are simultaneously associated to ATD.

Original languageEnglish (US)
Pages (from-to)783-790
Number of pages8
JournalClinical Biochemistry
Issue number9
Publication statusPublished - Jun 1 2009


All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

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