Set of secondary emission standards for calibration of the spectral responsivity in emission spectroscopy

J. A. Gardecki, Mark Maroncelli

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

Technical spectra are reported for a series of six secondary emission standards based on the commercially available fluorophors: tryptophan, α-NPO, tetraphenylbutadiene, coumarin 153, DCM, and LDS 751. These spectra cover the wavelength range between 300 and 800 nm with significant spectral overlap and conform to the requirements of secondary emission standards. Standard emission spectra are determined by averaging the technical spectra obtained from three independently calibrated fluorescence spectrometers. A method for generating the wavelength-dependent correction file by using these standards is outlined. Comparison to the National Bureau of Standards (NBS)-reported quinine sulfate spectrum illustrates the accuracy of both the emission standards and the method of generating a complete emission correction file. With the use of the standards and method reported here, the accuracy of the emission correction is estimated to be better than 10% for the entire wavelength range and significantly better over the wavelength region of a typical spectrum.

Original languageEnglish (US)
Pages (from-to)1179-1189
Number of pages11
JournalApplied Spectroscopy
Volume52
Issue number9
DOIs
StatePublished - Jan 1 1998

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Secondary emission
Emission spectroscopy
secondary emission
Calibration
spectroscopy
Wavelength
files
wavelengths
Quinine
tryptophan
Tryptophan
Spectrometers
sulfates
emission spectra
Fluorescence
spectrometers
fluorescence
requirements

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Spectroscopy

Cite this

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abstract = "Technical spectra are reported for a series of six secondary emission standards based on the commercially available fluorophors: tryptophan, α-NPO, tetraphenylbutadiene, coumarin 153, DCM, and LDS 751. These spectra cover the wavelength range between 300 and 800 nm with significant spectral overlap and conform to the requirements of secondary emission standards. Standard emission spectra are determined by averaging the technical spectra obtained from three independently calibrated fluorescence spectrometers. A method for generating the wavelength-dependent correction file by using these standards is outlined. Comparison to the National Bureau of Standards (NBS)-reported quinine sulfate spectrum illustrates the accuracy of both the emission standards and the method of generating a complete emission correction file. With the use of the standards and method reported here, the accuracy of the emission correction is estimated to be better than 10{\%} for the entire wavelength range and significantly better over the wavelength region of a typical spectrum.",
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Set of secondary emission standards for calibration of the spectral responsivity in emission spectroscopy. / Gardecki, J. A.; Maroncelli, Mark.

In: Applied Spectroscopy, Vol. 52, No. 9, 01.01.1998, p. 1179-1189.

Research output: Contribution to journalArticle

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AU - Maroncelli, Mark

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