Short communication: Comparison of 3 solid digesta passage markers in dairy cows

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study investigated the usefulness of acid-detergent fiber-bound 15N [acid detergent insoluble (ADI)-15N] as a solid digesta passage marker in dairy cows compared with chromium (Cr) and ytterbium (Yb) (as labeled fiber or forage, respectively). Intrinsically (ADI-15N) or extrinsically (Cr, Yb) labeled alfalfa hay was pulse-dosed intraruminally to 7 lactating dairy cows. Following marker administration, spot fecal samples were collected for up to 72h for marker analyses. Urine and milk samples were also collected and analyzed for Yb and Cr. Fecal marker excretion data were processed using 2-compartment mathematical age-dependent/age-independent (Gn→G1) models. The rate of passage of the marker in the first, age-dependent compartment tended to be slower for Yb compared with Cr and ADI-15N, which resulted in a trend for longer mean retention time (MRT) in this compartment when Yb was used as a marker (19.0h) compared with Cr and ADI-15N (14.5 and 13.9h, respectively). The rate constant of marker disappearance for the second or age-independent compartment tended to be greater for Yb compared with Cr and ADI-15N, which led to a shorter MRT of Yb in this compartment (15.6) versus ADI-15N (32.1) and Cr (24.8h). The cumulative MRT was greater for ADI-15N versus Cr and Yb (46.0, 39.3, and 34.4h, respectively). Total MRT of marker tended to be greater for ADI-15N than for Yb (46.6 vs. 36.6h, respectively). Urine and milk analyses data suggested no measurable losses of Yb along the digestive tract, but about 0.79% of Cr dosed intraruminally was secreted or excreted in milk and urine in the 48-h period following marker administration. Collectively, this study confirmed previous observations that ADI-15N can be used reliably as a solid digesta passage marker for ruminants, producing pre-duodenal and total-tract retention times similar to that of Cr-labeled fiber. Retention time in the age-independent compartment was underestimated when Yb was used as a marker, emphasizing the need to process forages to isolate fiber before labeling with Yb.

Original languageEnglish (US)
Pages (from-to)1725-1729
Number of pages5
JournalJournal of dairy science
Volume97
Issue number3
DOIs
StatePublished - Mar 1 2014

Fingerprint

Ytterbium
ytterbium
digesta
animal communication
Chromium
chromium
Detergents
dairy cows
detergents
Acids
acids
Milk
urine
Urine
milk
forage
Medicago sativa
Ruminants
alfalfa hay
acid detergent fiber

All Science Journal Classification (ASJC) codes

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

@article{fd08802bbba54b96847fae1658694e3c,
title = "Short communication: Comparison of 3 solid digesta passage markers in dairy cows",
abstract = "This study investigated the usefulness of acid-detergent fiber-bound 15N [acid detergent insoluble (ADI)-15N] as a solid digesta passage marker in dairy cows compared with chromium (Cr) and ytterbium (Yb) (as labeled fiber or forage, respectively). Intrinsically (ADI-15N) or extrinsically (Cr, Yb) labeled alfalfa hay was pulse-dosed intraruminally to 7 lactating dairy cows. Following marker administration, spot fecal samples were collected for up to 72h for marker analyses. Urine and milk samples were also collected and analyzed for Yb and Cr. Fecal marker excretion data were processed using 2-compartment mathematical age-dependent/age-independent (Gn→G1) models. The rate of passage of the marker in the first, age-dependent compartment tended to be slower for Yb compared with Cr and ADI-15N, which resulted in a trend for longer mean retention time (MRT) in this compartment when Yb was used as a marker (19.0h) compared with Cr and ADI-15N (14.5 and 13.9h, respectively). The rate constant of marker disappearance for the second or age-independent compartment tended to be greater for Yb compared with Cr and ADI-15N, which led to a shorter MRT of Yb in this compartment (15.6) versus ADI-15N (32.1) and Cr (24.8h). The cumulative MRT was greater for ADI-15N versus Cr and Yb (46.0, 39.3, and 34.4h, respectively). Total MRT of marker tended to be greater for ADI-15N than for Yb (46.6 vs. 36.6h, respectively). Urine and milk analyses data suggested no measurable losses of Yb along the digestive tract, but about 0.79{\%} of Cr dosed intraruminally was secreted or excreted in milk and urine in the 48-h period following marker administration. Collectively, this study confirmed previous observations that ADI-15N can be used reliably as a solid digesta passage marker for ruminants, producing pre-duodenal and total-tract retention times similar to that of Cr-labeled fiber. Retention time in the age-independent compartment was underestimated when Yb was used as a marker, emphasizing the need to process forages to isolate fiber before labeling with Yb.",
author = "C. Lee and Hristov, {Alexander Nikolov}",
year = "2014",
month = "3",
day = "1",
doi = "10.3168/jds.2013-7129",
language = "English (US)",
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Short communication : Comparison of 3 solid digesta passage markers in dairy cows. / Lee, C.; Hristov, Alexander Nikolov.

In: Journal of dairy science, Vol. 97, No. 3, 01.03.2014, p. 1725-1729.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Short communication

T2 - Comparison of 3 solid digesta passage markers in dairy cows

AU - Lee, C.

AU - Hristov, Alexander Nikolov

PY - 2014/3/1

Y1 - 2014/3/1

N2 - This study investigated the usefulness of acid-detergent fiber-bound 15N [acid detergent insoluble (ADI)-15N] as a solid digesta passage marker in dairy cows compared with chromium (Cr) and ytterbium (Yb) (as labeled fiber or forage, respectively). Intrinsically (ADI-15N) or extrinsically (Cr, Yb) labeled alfalfa hay was pulse-dosed intraruminally to 7 lactating dairy cows. Following marker administration, spot fecal samples were collected for up to 72h for marker analyses. Urine and milk samples were also collected and analyzed for Yb and Cr. Fecal marker excretion data were processed using 2-compartment mathematical age-dependent/age-independent (Gn→G1) models. The rate of passage of the marker in the first, age-dependent compartment tended to be slower for Yb compared with Cr and ADI-15N, which resulted in a trend for longer mean retention time (MRT) in this compartment when Yb was used as a marker (19.0h) compared with Cr and ADI-15N (14.5 and 13.9h, respectively). The rate constant of marker disappearance for the second or age-independent compartment tended to be greater for Yb compared with Cr and ADI-15N, which led to a shorter MRT of Yb in this compartment (15.6) versus ADI-15N (32.1) and Cr (24.8h). The cumulative MRT was greater for ADI-15N versus Cr and Yb (46.0, 39.3, and 34.4h, respectively). Total MRT of marker tended to be greater for ADI-15N than for Yb (46.6 vs. 36.6h, respectively). Urine and milk analyses data suggested no measurable losses of Yb along the digestive tract, but about 0.79% of Cr dosed intraruminally was secreted or excreted in milk and urine in the 48-h period following marker administration. Collectively, this study confirmed previous observations that ADI-15N can be used reliably as a solid digesta passage marker for ruminants, producing pre-duodenal and total-tract retention times similar to that of Cr-labeled fiber. Retention time in the age-independent compartment was underestimated when Yb was used as a marker, emphasizing the need to process forages to isolate fiber before labeling with Yb.

AB - This study investigated the usefulness of acid-detergent fiber-bound 15N [acid detergent insoluble (ADI)-15N] as a solid digesta passage marker in dairy cows compared with chromium (Cr) and ytterbium (Yb) (as labeled fiber or forage, respectively). Intrinsically (ADI-15N) or extrinsically (Cr, Yb) labeled alfalfa hay was pulse-dosed intraruminally to 7 lactating dairy cows. Following marker administration, spot fecal samples were collected for up to 72h for marker analyses. Urine and milk samples were also collected and analyzed for Yb and Cr. Fecal marker excretion data were processed using 2-compartment mathematical age-dependent/age-independent (Gn→G1) models. The rate of passage of the marker in the first, age-dependent compartment tended to be slower for Yb compared with Cr and ADI-15N, which resulted in a trend for longer mean retention time (MRT) in this compartment when Yb was used as a marker (19.0h) compared with Cr and ADI-15N (14.5 and 13.9h, respectively). The rate constant of marker disappearance for the second or age-independent compartment tended to be greater for Yb compared with Cr and ADI-15N, which led to a shorter MRT of Yb in this compartment (15.6) versus ADI-15N (32.1) and Cr (24.8h). The cumulative MRT was greater for ADI-15N versus Cr and Yb (46.0, 39.3, and 34.4h, respectively). Total MRT of marker tended to be greater for ADI-15N than for Yb (46.6 vs. 36.6h, respectively). Urine and milk analyses data suggested no measurable losses of Yb along the digestive tract, but about 0.79% of Cr dosed intraruminally was secreted or excreted in milk and urine in the 48-h period following marker administration. Collectively, this study confirmed previous observations that ADI-15N can be used reliably as a solid digesta passage marker for ruminants, producing pre-duodenal and total-tract retention times similar to that of Cr-labeled fiber. Retention time in the age-independent compartment was underestimated when Yb was used as a marker, emphasizing the need to process forages to isolate fiber before labeling with Yb.

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