Short-term in vitro culture of field isolates of Plasmodium vivax using umbilical cord blood

Rachanee Udomsangpetch, Sangdao Somsri, Tasanee Panichakul, Kesinee Chotivanich, Jeeraphat Sirichaisinthop, Zhaoqing Yang, Liwang Cui, Jetsumon Sattabongkot

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54 Scopus citations

Abstract

Plasmodium vivax research has been hampered by the lack of technology for culturing this parasite. Culturing P. vivax is difficult because the parasite selectively invades reticulocytes. Here we describe a modified procedure to establish and maintain short-term cultures of freshly collected P. vivax parasites using reticulocyte-enriched cord blood. Using this method, parasites could be cultured for a month. Manipulation of the culture allowed procurement of synchronized stages of the parasite. This short-term culture method can be easily adapted to study various aspects of the parasite biology.

Original languageEnglish (US)
Pages (from-to)65-69
Number of pages5
JournalParasitology International
Volume56
Issue number1
DOIs
StatePublished - Mar 1 2007

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

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    Udomsangpetch, R., Somsri, S., Panichakul, T., Chotivanich, K., Sirichaisinthop, J., Yang, Z., Cui, L., & Sattabongkot, J. (2007). Short-term in vitro culture of field isolates of Plasmodium vivax using umbilical cord blood. Parasitology International, 56(1), 65-69. https://doi.org/10.1016/j.parint.2006.12.005