Significance of EGFR protein expression and gene amplification in non-small cell lung carcinoma

Sanja Dacic, Melina Flanagan, Kathleen Cieply, Suresh Ramalingam, James Luketich, Chandra Belani, Samuel A. Yousem

Research output: Contribution to journalArticle

105 Scopus citations

Abstract

We evaluated epidermal growth factor receptor (EGFR) protein expression by immunohistochemical analysis and EGFR gene amplification by fluorescence in situ hybridization in 199 consecutive newly diagnosed and surgically treated patients with primary non-small cell lung carcinoma (NSCLC) and correlated results with clinicopathologic findings. EGFR protein expression was more common in squamous cell carcinoma (SCC; 17 [26.2%]) than in adenocarcinoma (14 [11.1%]; P = .0076) and more frequently associated with EGFR amplification (8 [14.5%] vs 4 [3.6%] cases; P = .0208). Poor differentiation was associated with a higher average number of EGFR gene copies per cell (mean, 4.18; P = .0322) and a higher EGFR/chromosome 7 ratio (mean, 1.84; P = .0324). NO disease showed a higher number of EGFR gene copies (mean, 4.196; P = .0163). SCCs demonstrated a higher EGFR/chromosome 7 ratio than adenocarcinomas (mean, 1.95 vs 1.47; P = .0324), particularly T1 tumors (mean, 1.79; P = .0243). Statistical analysis failed to show correlation between outcome and EGFR protein expression and gene amplification in early NSCLC. EGFR protein expression was uncoupled from gene amplification in most cases, although good correlation occurred in a subset of SCCs.

Original languageEnglish (US)
Pages (from-to)860-865
Number of pages6
JournalAmerican journal of clinical pathology
Volume125
Issue number6
DOIs
StatePublished - Jun 2006

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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