Silica-coated, Au/Ag striped nanowires for bioanalysis

James A. Sioss, Rebecca L. Stoermer, Michael Y. Sha, Christine Dolan Keating

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Striped metallic nanowires (NW) have been coated with a silica shell of controllable thickness (6-150 nm), and the assay performance of coated vs uncoated NW has been compared. The silica coating does not interfere with identification of the metal striping pattern and protects Ag segments from oxidation, extending the range of assay conditions under which barcoded NW can be used. Much higher and more uniform fluorescence intensities were observed for dye-labeled ssDNA bound to SiO 2-coated as compared to intensities for uncoated NW. Simultaneous, multiplexed DNA hybridization assays for three pathogen-specific target sequences on SiO 2-coated NW showed good discrimination of complementary from noncomplementary targets. Application of SiO 2-coated NW in discrimination of single base mismatches corresponding to a mutation of the p53 gene was also demonstrated. Finally, we have shown that thiolated probe DNA resists desorption under thermocycling conditions if attached via siloxane chemistry to SiO 2-coated NW, but not if it is attached via direct adsorption to bare Au/Ag NW.

Original languageEnglish (US)
Pages (from-to)11334-11341
Number of pages8
JournalLangmuir
Volume23
Issue number22
DOIs
StatePublished - Oct 23 2007

Fingerprint

Silicon Dioxide
Nanowires
nanowires
Silica
silicon dioxide
Assays
discrimination
DNA
deoxyribonucleic acid
Siloxanes
pathogens
siloxanes
DNA Probes
Thermal cycling
Pathogens
mutations
genes
Desorption
Coloring Agents
Dyes

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Sioss, James A. ; Stoermer, Rebecca L. ; Sha, Michael Y. ; Keating, Christine Dolan. / Silica-coated, Au/Ag striped nanowires for bioanalysis. In: Langmuir. 2007 ; Vol. 23, No. 22. pp. 11334-11341.
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Sioss, JA, Stoermer, RL, Sha, MY & Keating, CD 2007, 'Silica-coated, Au/Ag striped nanowires for bioanalysis', Langmuir, vol. 23, no. 22, pp. 11334-11341. https://doi.org/10.1021/la7019846

Silica-coated, Au/Ag striped nanowires for bioanalysis. / Sioss, James A.; Stoermer, Rebecca L.; Sha, Michael Y.; Keating, Christine Dolan.

In: Langmuir, Vol. 23, No. 22, 23.10.2007, p. 11334-11341.

Research output: Contribution to journalArticle

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