Silicon-induced systemic defense responses in perennial ryegrass against infection by Magnaporthe oryzae

Alamgir Rahman, Christopher M. Wallis, Wakar Uddin

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Sustainable integrated disease management for gray leaf spot of perennial ryegrass may involve use of plant defense elicitors with compatible traditional fungicides to reduce disease incidence and severity. Silicon (Si) has previously been identified as a potential inducer or modulator of plant defenses against different fungal pathogens. To this end, perennial ryegrass was inoculated with the causal agent of gray leaf spot, Magnaporthe oryzae,when grown in soil that was nonamended or amended with three different levels of calcium silicate (1, 5, or 10 metric tons [t]/ha). When applied at a rate of 5 t/ha, calcium silicate was found to significantly suppress gray leaf spot in perennial ryegrass, including a significant reduction of disease incidence (39.5%) and disease severity (47.3%). Additional studies observed non-penetrated papillae or cell-wall appositions harboring callose, phenolic autofluorogens, and lignin-associated polyphenolic compounds in grass grown in the Si-amended soil. Regarding defense-associated enzyme levels, only following infection did grass grown in Si-amended soil exhibit greater activities of peroxidase and polyphenol oxidase than equivalent inoculated control plants. Also following infection with M. oryzae, grass levels of several phenolic acids, including chlorogenic acid and flavonoids, and relative expression levels of genes encoding phenylalanine ammonia lyase (PALa and PALb) and lipoxygenase (LOXa) significantly increased in Si-amended plants compared with that of nonamended control plants. These results suggest that Si-mediated increase of host defense responses to fungal pathogens in perennial ryegrass has a great potential to be part of an effective integrated disease management strategy against gray leaf spot development.

Original languageEnglish (US)
Pages (from-to)748-757
Number of pages10
JournalPHYTOPATHOLOGY
Volume105
Issue number6
DOIs
StatePublished - Jun 1 2015

Fingerprint

Magnaporthe oryzae
leaf spot
silicon
Lolium perenne
calcium silicate
infection
grasses
disease incidence
disease severity
disease control
soil
pathogens
callose
phenylalanine ammonia-lyase
chlorogenic acid
lipoxygenase
phenolic acids
catechol oxidase
fungicides
lignin

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

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title = "Silicon-induced systemic defense responses in perennial ryegrass against infection by Magnaporthe oryzae",
abstract = "Sustainable integrated disease management for gray leaf spot of perennial ryegrass may involve use of plant defense elicitors with compatible traditional fungicides to reduce disease incidence and severity. Silicon (Si) has previously been identified as a potential inducer or modulator of plant defenses against different fungal pathogens. To this end, perennial ryegrass was inoculated with the causal agent of gray leaf spot, Magnaporthe oryzae,when grown in soil that was nonamended or amended with three different levels of calcium silicate (1, 5, or 10 metric tons [t]/ha). When applied at a rate of 5 t/ha, calcium silicate was found to significantly suppress gray leaf spot in perennial ryegrass, including a significant reduction of disease incidence (39.5{\%}) and disease severity (47.3{\%}). Additional studies observed non-penetrated papillae or cell-wall appositions harboring callose, phenolic autofluorogens, and lignin-associated polyphenolic compounds in grass grown in the Si-amended soil. Regarding defense-associated enzyme levels, only following infection did grass grown in Si-amended soil exhibit greater activities of peroxidase and polyphenol oxidase than equivalent inoculated control plants. Also following infection with M. oryzae, grass levels of several phenolic acids, including chlorogenic acid and flavonoids, and relative expression levels of genes encoding phenylalanine ammonia lyase (PALa and PALb) and lipoxygenase (LOXa) significantly increased in Si-amended plants compared with that of nonamended control plants. These results suggest that Si-mediated increase of host defense responses to fungal pathogens in perennial ryegrass has a great potential to be part of an effective integrated disease management strategy against gray leaf spot development.",
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Silicon-induced systemic defense responses in perennial ryegrass against infection by Magnaporthe oryzae. / Rahman, Alamgir; Wallis, Christopher M.; Uddin, Wakar.

In: PHYTOPATHOLOGY, Vol. 105, No. 6, 01.06.2015, p. 748-757.

Research output: Contribution to journalArticle

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