Simple method for determining nucleobase pKa values by indirect labeling and demonstration of a pKa of neutrality in dsDNA

Ellen M. Moody, Trevor S. Brown, Philip C. Bevilacqua

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Phosphorothioates were substituted into double-stranded DNA to study protonated Class I A+·C basepairs by 31P NMR. The method was effective in reporting the A+·C pKa. pKa values near 7.0 were found with optimal nearest-neighbor partners. Such pKa values could expand the catalytic repertoire of nucleic acids.

Original languageEnglish (US)
Pages (from-to)10200-10201
Number of pages2
JournalJournal of the American Chemical Society
Issue number33
StatePublished - Aug 25 2004

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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