Simple method for determining nucleobase pKa values by indirect labeling and demonstration of a pKa of neutrality in dsDNA

Ellen M. Moody, Trevor S. Brown, Philip C. Bevilacqua

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Phosphorothioates were substituted into double-stranded DNA to study protonated Class I A+·C basepairs by 31P NMR. The method was effective in reporting the A+·C pKa. pKa values near 7.0 were found with optimal nearest-neighbor partners. Such pKa values could expand the catalytic repertoire of nucleic acids.

Original languageEnglish (US)
Pages (from-to)10200-10201
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number33
DOIs
Publication statusPublished - Aug 25 2004

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this