Simple oPSU broth-BAX® PCR-PicoGreen® system for rapid detection of Listeria monocytogenes in pasteurized milk and hot dogs

Sangeetha Parameswaran, Robert B. Guyer, Stephen J. Knabel

Research output: Contribution to journalArticle

Abstract

Factors that significantly affect BAX® PCR detection of Listeria monocytogenes from optimized Penn State University (oPSU) broth were identified and optimized. Concentration of PCR product was significantly reduced by BAX™ protease and significantly increased by eliminating the lysis step and directly diluting oPSU broth prior to PCR. A simple oPSU broth-BAX® PCR-PicoGreen® (PSU-BAX-PicoGreen) system was developed and compared with current methods for detecting low levels of L. monocytogenes in commercial milk and hot dogs. All 30 milk samples inoculated with 10-20 CFU per ml L. monocytogenes were positive by FDA, BAX® and PSU-BAX-PicoGreen methods and all 42 uninoculated milk samples were negative by all of the above methods. All 30 hot dog samples inoculated with 10-20 CFU/g L. monocytogenes were positive by the USDA and PSU-BAX-PicoGreen methods, however, 2 hot dog samples gave indeterminate results with the standard BAX® method. All 42 uninoculated hot dog samples were negative by USDA, 9 were indeterminate by BAX®and 2 were positive by PSU-BAX-PicoGreen. The PSU-BAX-PicoGreen system may provide a simple and accurate method for rapidly screening pasteurized foods for both injured and noninjured L. monocytogenes.

Original languageEnglish (US)
Pages (from-to)233-248
Number of pages16
JournalJournal of Food Safety
Volume23
Issue number4
DOIs
StatePublished - Dec 2003

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hot dogs
pasteurized milk
Listeria monocytogenes
Milk
Dogs
Polymerase Chain Reaction
United States Department of Agriculture
milk
USDA
sampling
methodology
PicoGreen
Peptide Hydrolases
proteinases
screening
Food

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Food Science
  • Microbiology

Cite this

Parameswaran, Sangeetha ; Guyer, Robert B. ; Knabel, Stephen J. / Simple oPSU broth-BAX® PCR-PicoGreen® system for rapid detection of Listeria monocytogenes in pasteurized milk and hot dogs. In: Journal of Food Safety. 2003 ; Vol. 23, No. 4. pp. 233-248.
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abstract = "Factors that significantly affect BAX{\circledR} PCR detection of Listeria monocytogenes from optimized Penn State University (oPSU) broth were identified and optimized. Concentration of PCR product was significantly reduced by BAX™ protease and significantly increased by eliminating the lysis step and directly diluting oPSU broth prior to PCR. A simple oPSU broth-BAX{\circledR} PCR-PicoGreen{\circledR} (PSU-BAX-PicoGreen) system was developed and compared with current methods for detecting low levels of L. monocytogenes in commercial milk and hot dogs. All 30 milk samples inoculated with 10-20 CFU per ml L. monocytogenes were positive by FDA, BAX{\circledR} and PSU-BAX-PicoGreen methods and all 42 uninoculated milk samples were negative by all of the above methods. All 30 hot dog samples inoculated with 10-20 CFU/g L. monocytogenes were positive by the USDA and PSU-BAX-PicoGreen methods, however, 2 hot dog samples gave indeterminate results with the standard BAX{\circledR} method. All 42 uninoculated hot dog samples were negative by USDA, 9 were indeterminate by BAX{\circledR}and 2 were positive by PSU-BAX-PicoGreen. The PSU-BAX-PicoGreen system may provide a simple and accurate method for rapidly screening pasteurized foods for both injured and noninjured L. monocytogenes.",
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Simple oPSU broth-BAX® PCR-PicoGreen® system for rapid detection of Listeria monocytogenes in pasteurized milk and hot dogs. / Parameswaran, Sangeetha; Guyer, Robert B.; Knabel, Stephen J.

In: Journal of Food Safety, Vol. 23, No. 4, 12.2003, p. 233-248.

Research output: Contribution to journalArticle

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