Simple Polymethylglutarimide Microfluidic Channels With Hydrogel-Assisted Fluid Exchange

Akanksha V. Gupta, Alyssa N. Brigeman, Esther W. Gomez, Thomas N. Jackson

Research output: Contribution to journalArticle

Abstract

We present an experimental protocol for fabricating enclosed microfluidic channels using polymethylglutarimide (PMGI). PMGI is optically transparent, biocompatible, and can be used to readily fabricate micrometer-scale lateral and vertical dimension channels using conventional photolithography. The low auto-fluorescence intensity of PMGI facilitates imaging of analytes without interference. The hydrophilicity of PMGI allows fluid exchange in micrometer-scale channels using a hydrogel as an interface without an external pump. As a demonstration, we assemble fluorescently-labeled lipid bilayers in PMGI microfluidic channels and show that PMGI has negligible auto-fluorescence intensity compared to the lipid bilayer. PMGI channels together with hydrogel-assisted fluidic exchange provides a simple approach to fabricate micrometer and sub-micrometer scale fluidic channels for optofluidics, molecular biology, and other medical diagnostic and sensing applications.

Original languageEnglish (US)
Pages (from-to)97-101
Number of pages5
JournalIEEE Transactions on Nanobioscience
Volume17
Issue number2
DOIs
StatePublished - Apr 2018

Fingerprint

Microfluidics
Hydrogel
Lipid Bilayers
Hydrogels
Fluorescence
Vertical Dimension
Lipid bilayers
Fluids
Fluidics
Hydrophobic and Hydrophilic Interactions
Molecular Biology
Molecular biology
Hydrophilicity
Photolithography
Demonstrations
Pumps
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmaceutical Science
  • Computer Science Applications
  • Electrical and Electronic Engineering

Cite this

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Simple Polymethylglutarimide Microfluidic Channels With Hydrogel-Assisted Fluid Exchange. / Gupta, Akanksha V.; Brigeman, Alyssa N.; Gomez, Esther W.; Jackson, Thomas N.

In: IEEE Transactions on Nanobioscience, Vol. 17, No. 2, 04.2018, p. 97-101.

Research output: Contribution to journalArticle

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