Simultaneous cellulose degradation and electricity production by Enterobacter cloacae in a microbial fuel cell

Farzaneh Rezaei, Defeng Xing, Rachel Wagner, John M. Regan, Tom L. Richard, Bruce E. Logan

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

Electricity can be directly generated by bacteria in microbial fuel cells (MFCs) from many different biodegradable substrates. When cellulose is used as the substrate, electricity generation requires a microbial community with both cellulolytic and exoelectrogenic activities. Cellulose degradation with electricity production by a pure culture has not been previously demonstrated without addition of an exogenous mediator. Using a specially designed U-tube MFC, we enriched a consortium of exoelectrogenic bacteria capable of using cellulose as the sole electron donor. After 19 dilution-to-extinction serial transfers of the consortium, 16S rRNA gene-based community analysis using denaturing gradient gel electrophoresis and band sequencing revealed that the dominant bacterium was Enterobacter cloacae. An isolate designated E. cloacae FR from the enrichment was found to be 100% identical to E. cloacae ATCC 13047T based on a partial 16S rRNA sequence. In polarization tests using the U-tube MFC and cellulose as a substrate, strain FR produced 4.9 ± 0.01 mW/m2, compared to 5.4 ± 0.3 mW/m2 for strain ATCC 13047T. These results demonstrate for the first time that it is possible to generate electricity from cellulose using a single bacterial strain without exogenous mediators.

Original languageEnglish (US)
Pages (from-to)3673-3678
Number of pages6
JournalApplied and environmental microbiology
Volume75
Issue number11
DOIs
StatePublished - Jun 1 2009

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Bioelectric Energy Sources
microbial fuel cells
Enterobacter cloacae
Electricity
electricity
fuel cell
Cellulose
cellulose
degradation
Bacteria
substrate
bacterium
bacteria
ribosomal RNA
Denaturing Gradient Gel Electrophoresis
biodegradability
electricity generation
denaturing gradient gel electrophoresis
rRNA Genes
microbial communities

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

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abstract = "Electricity can be directly generated by bacteria in microbial fuel cells (MFCs) from many different biodegradable substrates. When cellulose is used as the substrate, electricity generation requires a microbial community with both cellulolytic and exoelectrogenic activities. Cellulose degradation with electricity production by a pure culture has not been previously demonstrated without addition of an exogenous mediator. Using a specially designed U-tube MFC, we enriched a consortium of exoelectrogenic bacteria capable of using cellulose as the sole electron donor. After 19 dilution-to-extinction serial transfers of the consortium, 16S rRNA gene-based community analysis using denaturing gradient gel electrophoresis and band sequencing revealed that the dominant bacterium was Enterobacter cloacae. An isolate designated E. cloacae FR from the enrichment was found to be 100{\%} identical to E. cloacae ATCC 13047T based on a partial 16S rRNA sequence. In polarization tests using the U-tube MFC and cellulose as a substrate, strain FR produced 4.9 ± 0.01 mW/m2, compared to 5.4 ± 0.3 mW/m2 for strain ATCC 13047T. These results demonstrate for the first time that it is possible to generate electricity from cellulose using a single bacterial strain without exogenous mediators.",
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Simultaneous cellulose degradation and electricity production by Enterobacter cloacae in a microbial fuel cell. / Rezaei, Farzaneh; Xing, Defeng; Wagner, Rachel; Regan, John M.; Richard, Tom L.; Logan, Bruce E.

In: Applied and environmental microbiology, Vol. 75, No. 11, 01.06.2009, p. 3673-3678.

Research output: Contribution to journalArticle

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