TY - JOUR
T1 - Simultaneous Counting of Molecules in the Halo and Dense-Core of Nanovesicles by Regulating Dynamics of Vesicle Opening
AU - He, Xiulan
AU - Ewing, Andrew G.
N1 - Funding Information:
The European Research Council (ERC Advanced Grant Project No 787534 NanoBioNext), Knut and Alice Wallenberg Foundation, and the Swedish Research Council (VR Grant No 2017‐04366, 2017‐05962, 2020‐00803) are acknowledged for financial support. We would like to thank Dalsjöfors Kött AB, Sweden, and their employees for their kind help with providing the adrenal glands.
Publisher Copyright:
© 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.
PY - 2022/4/4
Y1 - 2022/4/4
N2 - We report the discovery that in the presence of chaotropic anions (SCN−) the opening of nanometer biological vesicles at an electrified interface often becomes a two-step process (around 30 % doublet peaks). We have then used this to independently count molecules in each subvesicular compartment, the halo and protein dense-core, and the fraction of catecholamine binding to the dense-core is 68 %. Moreover, we differentiated two distinct populations of large dense-core vesicles (LDCVs) and quantified their content, which might correspond to immature (43 %) and mature (30 %) LDCVs, to reveal differences in their biogenesis. We speculate this is caused by an increase in the electrostatic attraction between protonated catecholamine and the negatively charged dense-core following adsorption of SCN−.
AB - We report the discovery that in the presence of chaotropic anions (SCN−) the opening of nanometer biological vesicles at an electrified interface often becomes a two-step process (around 30 % doublet peaks). We have then used this to independently count molecules in each subvesicular compartment, the halo and protein dense-core, and the fraction of catecholamine binding to the dense-core is 68 %. Moreover, we differentiated two distinct populations of large dense-core vesicles (LDCVs) and quantified their content, which might correspond to immature (43 %) and mature (30 %) LDCVs, to reveal differences in their biogenesis. We speculate this is caused by an increase in the electrostatic attraction between protonated catecholamine and the negatively charged dense-core following adsorption of SCN−.
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U2 - 10.1002/anie.202116217
DO - 10.1002/anie.202116217
M3 - Article
C2 - 35129861
AN - SCOPUS:85124760089
SN - 1433-7851
VL - 61
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 15
M1 - e202116217
ER -