Simultaneous determination of thioridazine and its S-oxidized and N-demethylated metabolites using high-performance liquid chromatography on radially compressed silica

Clinton D. Kilts, Kennerly S. Patrick, George R. Breese, Richard B. Mailman

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Abstract

A method for simultaneously quantifying thioridazine, northioridazine, thioridazine-2-sulfoxide, thioridazine-2-sulfone and thioridazine-5-oxide in serum and plasma is described. Following solvent extraction these compounds were separated by high-performance liquid chromatography on radially compressed silica gel and detected by UV absorbance at 254 nm. Chromatography time is less than 7 min. The relative retention of these compounds as a function of the methanol and methylamine content of the mobile phase is discussed. Practical limits of detection, based upon an assayed plasma or serum volume of 1 ml, were 20 ng/ml for thioridazine-5-oxide and 10 ng/ml for the other compounds. The coefficient of variation for all compounds was less than 13%. The method is compared with more conventional high-performance liquid chromatographic and gas chromatographic methodology.

Original languageEnglish (US)
Pages (from-to)377-391
Number of pages15
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume231
Issue number2
DOIs
StatePublished - Sep 10 1982

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All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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