Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots

Daniel Gonzalez, Chiara Melloni, Brenda B. Poindexter, Ram Yogev, Andrew M. Atz, Janice E. Sullivan, Susan R. Mendley, Paula Delmore, Amy Delinsky, Kanecia Zimmerman, Andrew Lewandowski, Barrie Harper, Kenneth C. Lewis, Daniel K. Benjamin, Michael Cohen-Wolkowiez, K. Y. Berezny, E. Capparelli, G. L. Kearns, M. Laughon, A. Muelenaer & 24 others T. M. O'Shea, Ian Paul, P. B. Smith, J. Van Den Anker, K. Wade, T. J. Walsh, D. Siegel, P. Taylor-Zapata, A. Zajicek, Z. Ren, K. Tsilou, A. Pagan, R. Anand, G. Simone, A. Lewandowski, B. Harper, B. Poindexter, L. Smiley, R. Yogev, L. Fern, H. Al Nasiri, J. Sullivan, T. Bratton, Donna Cannonier

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Trimethoprim-sulfamethoxazole (TMP-SMX) is an antimicrobial drug combination commonly prescribed in children and adults. The study objectives were to validate and apply an HPLC-MS/MS method to quantify TMP-SMX in dried plasma spots (DPS) and dried urine spots (DUS), and perform a comparability analysis with liquid matrices. Results: For TMP the validated range was 100-50,000 ng/ml for DPS and 500-250,000 ng/ml for DUS; for SMX, the validated range was 1000-500,000 ng/ml for both DPS and DUS. Good agreement was noted between DPS/DUS and liquid plasma and urine samples for TMP, while only modest agreement was observed for SMX in both matrices. Conclusion: A precise, accurate and reproducible method was developed to quantify TMP-SMX in DPS and DUS samples.

Original languageEnglish (US)
Pages (from-to)1137-1149
Number of pages13
JournalBioanalysis
Volume7
Issue number9
DOIs
StatePublished - May 1 2015

Fingerprint

Sulfamethoxazole Drug Combination Trimethoprim
Urine
Plasmas
Thymidine Monophosphate
Liquids
Drug Combinations
High Pressure Liquid Chromatography

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Clinical Biochemistry
  • Medical Laboratory Technology

Cite this

Gonzalez, D., Melloni, C., Poindexter, B. B., Yogev, R., Atz, A. M., Sullivan, J. E., ... Cannonier, D. (2015). Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots. Bioanalysis, 7(9), 1137-1149. https://doi.org/10.4155/bio.15.38
Gonzalez, Daniel ; Melloni, Chiara ; Poindexter, Brenda B. ; Yogev, Ram ; Atz, Andrew M. ; Sullivan, Janice E. ; Mendley, Susan R. ; Delmore, Paula ; Delinsky, Amy ; Zimmerman, Kanecia ; Lewandowski, Andrew ; Harper, Barrie ; Lewis, Kenneth C. ; Benjamin, Daniel K. ; Cohen-Wolkowiez, Michael ; Berezny, K. Y. ; Capparelli, E. ; Kearns, G. L. ; Laughon, M. ; Muelenaer, A. ; O'Shea, T. M. ; Paul, Ian ; Smith, P. B. ; Van Den Anker, J. ; Wade, K. ; Walsh, T. J. ; Siegel, D. ; Taylor-Zapata, P. ; Zajicek, A. ; Ren, Z. ; Tsilou, K. ; Pagan, A. ; Anand, R. ; Simone, G. ; Lewandowski, A. ; Harper, B. ; Poindexter, B. ; Smiley, L. ; Yogev, R. ; Fern, L. ; Al Nasiri, H. ; Sullivan, J. ; Bratton, T. ; Cannonier, Donna. / Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots. In: Bioanalysis. 2015 ; Vol. 7, No. 9. pp. 1137-1149.
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abstract = "Background: Trimethoprim-sulfamethoxazole (TMP-SMX) is an antimicrobial drug combination commonly prescribed in children and adults. The study objectives were to validate and apply an HPLC-MS/MS method to quantify TMP-SMX in dried plasma spots (DPS) and dried urine spots (DUS), and perform a comparability analysis with liquid matrices. Results: For TMP the validated range was 100-50,000 ng/ml for DPS and 500-250,000 ng/ml for DUS; for SMX, the validated range was 1000-500,000 ng/ml for both DPS and DUS. Good agreement was noted between DPS/DUS and liquid plasma and urine samples for TMP, while only modest agreement was observed for SMX in both matrices. Conclusion: A precise, accurate and reproducible method was developed to quantify TMP-SMX in DPS and DUS samples.",
author = "Daniel Gonzalez and Chiara Melloni and Poindexter, {Brenda B.} and Ram Yogev and Atz, {Andrew M.} and Sullivan, {Janice E.} and Mendley, {Susan R.} and Paula Delmore and Amy Delinsky and Kanecia Zimmerman and Andrew Lewandowski and Barrie Harper and Lewis, {Kenneth C.} and Benjamin, {Daniel K.} and Michael Cohen-Wolkowiez and Berezny, {K. Y.} and E. Capparelli and Kearns, {G. L.} and M. Laughon and A. Muelenaer and O'Shea, {T. M.} and Ian Paul and Smith, {P. B.} and {Van Den Anker}, J. and K. Wade and Walsh, {T. J.} and D. Siegel and P. Taylor-Zapata and A. Zajicek and Z. Ren and K. Tsilou and A. Pagan and R. Anand and G. Simone and A. Lewandowski and B. Harper and B. Poindexter and L. Smiley and R. Yogev and L. Fern and {Al Nasiri}, H. and J. Sullivan and T. Bratton and Donna Cannonier",
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Gonzalez, D, Melloni, C, Poindexter, BB, Yogev, R, Atz, AM, Sullivan, JE, Mendley, SR, Delmore, P, Delinsky, A, Zimmerman, K, Lewandowski, A, Harper, B, Lewis, KC, Benjamin, DK, Cohen-Wolkowiez, M, Berezny, KY, Capparelli, E, Kearns, GL, Laughon, M, Muelenaer, A, O'Shea, TM, Paul, I, Smith, PB, Van Den Anker, J, Wade, K, Walsh, TJ, Siegel, D, Taylor-Zapata, P, Zajicek, A, Ren, Z, Tsilou, K, Pagan, A, Anand, R, Simone, G, Lewandowski, A, Harper, B, Poindexter, B, Smiley, L, Yogev, R, Fern, L, Al Nasiri, H, Sullivan, J, Bratton, T & Cannonier, D 2015, 'Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots', Bioanalysis, vol. 7, no. 9, pp. 1137-1149. https://doi.org/10.4155/bio.15.38

Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots. / Gonzalez, Daniel; Melloni, Chiara; Poindexter, Brenda B.; Yogev, Ram; Atz, Andrew M.; Sullivan, Janice E.; Mendley, Susan R.; Delmore, Paula; Delinsky, Amy; Zimmerman, Kanecia; Lewandowski, Andrew; Harper, Barrie; Lewis, Kenneth C.; Benjamin, Daniel K.; Cohen-Wolkowiez, Michael; Berezny, K. Y.; Capparelli, E.; Kearns, G. L.; Laughon, M.; Muelenaer, A.; O'Shea, T. M.; Paul, Ian; Smith, P. B.; Van Den Anker, J.; Wade, K.; Walsh, T. J.; Siegel, D.; Taylor-Zapata, P.; Zajicek, A.; Ren, Z.; Tsilou, K.; Pagan, A.; Anand, R.; Simone, G.; Lewandowski, A.; Harper, B.; Poindexter, B.; Smiley, L.; Yogev, R.; Fern, L.; Al Nasiri, H.; Sullivan, J.; Bratton, T.; Cannonier, Donna.

In: Bioanalysis, Vol. 7, No. 9, 01.05.2015, p. 1137-1149.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots

AU - Gonzalez, Daniel

AU - Melloni, Chiara

AU - Poindexter, Brenda B.

AU - Yogev, Ram

AU - Atz, Andrew M.

AU - Sullivan, Janice E.

AU - Mendley, Susan R.

AU - Delmore, Paula

AU - Delinsky, Amy

AU - Zimmerman, Kanecia

AU - Lewandowski, Andrew

AU - Harper, Barrie

AU - Lewis, Kenneth C.

AU - Benjamin, Daniel K.

AU - Cohen-Wolkowiez, Michael

AU - Berezny, K. Y.

AU - Capparelli, E.

AU - Kearns, G. L.

AU - Laughon, M.

AU - Muelenaer, A.

AU - O'Shea, T. M.

AU - Paul, Ian

AU - Smith, P. B.

AU - Van Den Anker, J.

AU - Wade, K.

AU - Walsh, T. J.

AU - Siegel, D.

AU - Taylor-Zapata, P.

AU - Zajicek, A.

AU - Ren, Z.

AU - Tsilou, K.

AU - Pagan, A.

AU - Anand, R.

AU - Simone, G.

AU - Lewandowski, A.

AU - Harper, B.

AU - Poindexter, B.

AU - Smiley, L.

AU - Yogev, R.

AU - Fern, L.

AU - Al Nasiri, H.

AU - Sullivan, J.

AU - Bratton, T.

AU - Cannonier, Donna

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Background: Trimethoprim-sulfamethoxazole (TMP-SMX) is an antimicrobial drug combination commonly prescribed in children and adults. The study objectives were to validate and apply an HPLC-MS/MS method to quantify TMP-SMX in dried plasma spots (DPS) and dried urine spots (DUS), and perform a comparability analysis with liquid matrices. Results: For TMP the validated range was 100-50,000 ng/ml for DPS and 500-250,000 ng/ml for DUS; for SMX, the validated range was 1000-500,000 ng/ml for both DPS and DUS. Good agreement was noted between DPS/DUS and liquid plasma and urine samples for TMP, while only modest agreement was observed for SMX in both matrices. Conclusion: A precise, accurate and reproducible method was developed to quantify TMP-SMX in DPS and DUS samples.

AB - Background: Trimethoprim-sulfamethoxazole (TMP-SMX) is an antimicrobial drug combination commonly prescribed in children and adults. The study objectives were to validate and apply an HPLC-MS/MS method to quantify TMP-SMX in dried plasma spots (DPS) and dried urine spots (DUS), and perform a comparability analysis with liquid matrices. Results: For TMP the validated range was 100-50,000 ng/ml for DPS and 500-250,000 ng/ml for DUS; for SMX, the validated range was 1000-500,000 ng/ml for both DPS and DUS. Good agreement was noted between DPS/DUS and liquid plasma and urine samples for TMP, while only modest agreement was observed for SMX in both matrices. Conclusion: A precise, accurate and reproducible method was developed to quantify TMP-SMX in DPS and DUS samples.

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Gonzalez D, Melloni C, Poindexter BB, Yogev R, Atz AM, Sullivan JE et al. Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots. Bioanalysis. 2015 May 1;7(9):1137-1149. https://doi.org/10.4155/bio.15.38