Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots

Daniel Gonzalez, Chiara Melloni, Brenda B. Poindexter, Ram Yogev, Andrew M. Atz, Janice E. Sullivan, Susan R. Mendley, Paula Delmore, Amy Delinsky, Kanecia Zimmerman, Andrew Lewandowski, Barrie Harper, Kenneth C. Lewis, Daniel K. Benjamin, Michael Cohen-Wolkowiez, K. Y. Berezny, E. Capparelli, G. L. Kearns, M. Laughon, A. MuelenaerT. M. O'Shea, I. M. Paul, P. B. Smith, J. Van Den Anker, K. Wade, T. J. Walsh, D. Siegel, P. Taylor-Zapata, A. Zajicek, Z. Ren, K. Tsilou, A. Pagan, R. Anand, G. Simone, A. Lewandowski, B. Harper, B. Poindexter, L. Smiley, R. Yogev, L. Fern, H. Al Nasiri, J. Sullivan, T. Bratton, Donna Cannonier

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Background: Trimethoprim-sulfamethoxazole (TMP-SMX) is an antimicrobial drug combination commonly prescribed in children and adults. The study objectives were to validate and apply an HPLC-MS/MS method to quantify TMP-SMX in dried plasma spots (DPS) and dried urine spots (DUS), and perform a comparability analysis with liquid matrices. Results: For TMP the validated range was 100-50,000 ng/ml for DPS and 500-250,000 ng/ml for DUS; for SMX, the validated range was 1000-500,000 ng/ml for both DPS and DUS. Good agreement was noted between DPS/DUS and liquid plasma and urine samples for TMP, while only modest agreement was observed for SMX in both matrices. Conclusion: A precise, accurate and reproducible method was developed to quantify TMP-SMX in DPS and DUS samples.

Original languageEnglish (US)
Pages (from-to)1137-1149
Number of pages13
JournalBioanalysis
Volume7
Issue number9
DOIs
StatePublished - May 1 2015

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Clinical Biochemistry
  • Medical Laboratory Technology

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    Gonzalez, D., Melloni, C., Poindexter, B. B., Yogev, R., Atz, A. M., Sullivan, J. E., Mendley, S. R., Delmore, P., Delinsky, A., Zimmerman, K., Lewandowski, A., Harper, B., Lewis, K. C., Benjamin, D. K., Cohen-Wolkowiez, M., Berezny, K. Y., Capparelli, E., Kearns, G. L., Laughon, M., ... Cannonier, D. (2015). Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots. Bioanalysis, 7(9), 1137-1149. https://doi.org/10.4155/bio.15.38