Simultaneous electrochemical and unit recording measurements: Characterization of the effects of d-amphetamine and ascorbic acid on neostriatal neurons

Andrew G. Ewing, Kevin D. Alloway, Stephen D. Curtis, Mark A. Dayton, R. Mark Wightman, George V. Rebec

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Abstract

Simultaneous electrochemical measurements and recordings of single-unit activity were obtained from the neostriatum of immobilized, locally anesthetized rats. Electrical cross-talk between the two systems was minimized by the use of carbon-fiber voltammetric electrodes, which generate very small currents during electrolysis, and by the careful isolation of the recording apparatus. An intraperitoneal (i.p.) injection of 2.5 mg/kg d-amphetamine (d-AMPH) typically inhibited unit activity and produced a corresponding mirror-image increase in oxidation current. Both the depression of firing rate and the elevated electrochemical signal were maximal approximately 25 min after d-AMPH administration. In contrast, both unit activity and oxidation current were elevated after administration of 7.5 mg/kg d-AMPH with the maximum change in firing rate occurring significantly sooner than the peak electrochemical response. In view of evidence that the d-AMPH-induced voltammetric signal is similar to that for ascorbic acid (AA), but not dopamine (DA), a separate group of 12 animals received 1000 mg/kg AA (i.p.). In all cases, AA increased the electrochemical signal and in 7 animals this response was accompanied by an increase in firing rate. Our results, which demonstrate the feasibility of combining in vivo electrochemistry with single-unit electrophysiology, suggest that a d-AMPH-induced release of DA cannot completely explain the dose-dependent biphasic shift in neuronal activity produced by this drug in the neostriatum. Moreover, AA appears to be an effective modulator of neostriatal activity.

Original languageEnglish (US)
Pages (from-to)101-108
Number of pages8
JournalBrain research
Volume261
Issue number1
DOIs
StatePublished - Feb 14 1983

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Dextroamphetamine
Ascorbic Acid
Neurons
Neostriatum
Dopamine
Electrolysis
Electrochemistry
Electrophysiology
Intraperitoneal Injections
Electrodes
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Ewing, Andrew G. ; Alloway, Kevin D. ; Curtis, Stephen D. ; Dayton, Mark A. ; Wightman, R. Mark ; Rebec, George V. / Simultaneous electrochemical and unit recording measurements : Characterization of the effects of d-amphetamine and ascorbic acid on neostriatal neurons. In: Brain research. 1983 ; Vol. 261, No. 1. pp. 101-108.
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Simultaneous electrochemical and unit recording measurements : Characterization of the effects of d-amphetamine and ascorbic acid on neostriatal neurons. / Ewing, Andrew G.; Alloway, Kevin D.; Curtis, Stephen D.; Dayton, Mark A.; Wightman, R. Mark; Rebec, George V.

In: Brain research, Vol. 261, No. 1, 14.02.1983, p. 101-108.

Research output: Contribution to journalArticle

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