Simultaneous online recovery of human lysozyme produced by kluyveromyces lactis K7 in biofilm reactor

Duygu Ercan, Ali Demirci

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Lysozyme is an antimicrobial compound, which has been used in both food and pharmaceutical industry. Kluyveromyces lactis K7 is a genetically modified microorganism, which is proposed to produce the human lysozyme commercially. Up to now, human lysozyme production was enhanced by the application of biofilm reactor with plastic composite (173 U/ml) compared to the suspended-cell bioreactor (110 U/ml). However, lysozyme has antimicrobial peptides, which might affect the growth of yeast cells during fermentation. As a result, high production potential of human lysozyme might be reduced. Therefore, a simultaneous fermentation and online recovery system can help to enhance the production. In order to test this strategy, the objective of this study was to select the best adsorption and desorption conditions for the resin, silicic acid, and evaluate the simultaneous fermentation and online recovery system for the production of human lysozyme by K. lactis K7 in biofilm reactor. The results demonstrated that the best adsorption (95.6% adsorption) was obtained at 25°C, pH 4, and 25% silicic acid ratio. Also, the best desorption parameters were determined as 25°C, pH -6.2, and 5% sodium dodecyl sulfate with 1 M NaCI and 20% ethanol as eluent composition. Four repetitive desorption process provided up to 98% desorption. Finally, the simultaneous fermentation and online recovery system with tangential flow microfilter module improved the production of human lysozyme to280.4 U/ml, which is 63% improvement compared to the biofilm bioreactor without online recovery system.

Original languageEnglish (US)
Title of host publicationAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014
PublisherAmerican Society of Agricultural and Biological Engineers
Pages1318-1330
Number of pages13
ISBN (Electronic)9781632668455
StatePublished - Jan 1 2014
EventAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014 - Montreal, Canada
Duration: Jul 13 2014Jul 16 2014

Publication series

NameAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014
Volume2

Other

OtherAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014
CountryCanada
CityMontreal
Period7/13/147/16/14

Fingerprint

Kluyveromyces
Kluyveromyces marxianus var. lactis
Biofilms
Muramidase
lysozyme
biofilm
Enzymes
Online Systems
Recovery
desorption
Fermentation
Desorption
fermentation
Silicic Acid
Adsorption
silicic acid
adsorption
Bioreactors
bioreactors
genetically engineered microorganisms

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Mechanical Engineering

Cite this

Ercan, D., & Demirci, A. (2014). Simultaneous online recovery of human lysozyme produced by kluyveromyces lactis K7 in biofilm reactor. In American Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014 (pp. 1318-1330). (American Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014; Vol. 2). American Society of Agricultural and Biological Engineers.
Ercan, Duygu ; Demirci, Ali. / Simultaneous online recovery of human lysozyme produced by kluyveromyces lactis K7 in biofilm reactor. American Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014. American Society of Agricultural and Biological Engineers, 2014. pp. 1318-1330 (American Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014).
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title = "Simultaneous online recovery of human lysozyme produced by kluyveromyces lactis K7 in biofilm reactor",
abstract = "Lysozyme is an antimicrobial compound, which has been used in both food and pharmaceutical industry. Kluyveromyces lactis K7 is a genetically modified microorganism, which is proposed to produce the human lysozyme commercially. Up to now, human lysozyme production was enhanced by the application of biofilm reactor with plastic composite (173 U/ml) compared to the suspended-cell bioreactor (110 U/ml). However, lysozyme has antimicrobial peptides, which might affect the growth of yeast cells during fermentation. As a result, high production potential of human lysozyme might be reduced. Therefore, a simultaneous fermentation and online recovery system can help to enhance the production. In order to test this strategy, the objective of this study was to select the best adsorption and desorption conditions for the resin, silicic acid, and evaluate the simultaneous fermentation and online recovery system for the production of human lysozyme by K. lactis K7 in biofilm reactor. The results demonstrated that the best adsorption (95.6{\%} adsorption) was obtained at 25°C, pH 4, and 25{\%} silicic acid ratio. Also, the best desorption parameters were determined as 25°C, pH -6.2, and 5{\%} sodium dodecyl sulfate with 1 M NaCI and 20{\%} ethanol as eluent composition. Four repetitive desorption process provided up to 98{\%} desorption. Finally, the simultaneous fermentation and online recovery system with tangential flow microfilter module improved the production of human lysozyme to280.4 U/ml, which is 63{\%} improvement compared to the biofilm bioreactor without online recovery system.",
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Ercan, D & Demirci, A 2014, Simultaneous online recovery of human lysozyme produced by kluyveromyces lactis K7 in biofilm reactor. in American Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014. American Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014, vol. 2, American Society of Agricultural and Biological Engineers, pp. 1318-1330, American Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014, Montreal, Canada, 7/13/14.

Simultaneous online recovery of human lysozyme produced by kluyveromyces lactis K7 in biofilm reactor. / Ercan, Duygu; Demirci, Ali.

American Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014. American Society of Agricultural and Biological Engineers, 2014. p. 1318-1330 (American Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Demirci, Ali

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N2 - Lysozyme is an antimicrobial compound, which has been used in both food and pharmaceutical industry. Kluyveromyces lactis K7 is a genetically modified microorganism, which is proposed to produce the human lysozyme commercially. Up to now, human lysozyme production was enhanced by the application of biofilm reactor with plastic composite (173 U/ml) compared to the suspended-cell bioreactor (110 U/ml). However, lysozyme has antimicrobial peptides, which might affect the growth of yeast cells during fermentation. As a result, high production potential of human lysozyme might be reduced. Therefore, a simultaneous fermentation and online recovery system can help to enhance the production. In order to test this strategy, the objective of this study was to select the best adsorption and desorption conditions for the resin, silicic acid, and evaluate the simultaneous fermentation and online recovery system for the production of human lysozyme by K. lactis K7 in biofilm reactor. The results demonstrated that the best adsorption (95.6% adsorption) was obtained at 25°C, pH 4, and 25% silicic acid ratio. Also, the best desorption parameters were determined as 25°C, pH -6.2, and 5% sodium dodecyl sulfate with 1 M NaCI and 20% ethanol as eluent composition. Four repetitive desorption process provided up to 98% desorption. Finally, the simultaneous fermentation and online recovery system with tangential flow microfilter module improved the production of human lysozyme to280.4 U/ml, which is 63% improvement compared to the biofilm bioreactor without online recovery system.

AB - Lysozyme is an antimicrobial compound, which has been used in both food and pharmaceutical industry. Kluyveromyces lactis K7 is a genetically modified microorganism, which is proposed to produce the human lysozyme commercially. Up to now, human lysozyme production was enhanced by the application of biofilm reactor with plastic composite (173 U/ml) compared to the suspended-cell bioreactor (110 U/ml). However, lysozyme has antimicrobial peptides, which might affect the growth of yeast cells during fermentation. As a result, high production potential of human lysozyme might be reduced. Therefore, a simultaneous fermentation and online recovery system can help to enhance the production. In order to test this strategy, the objective of this study was to select the best adsorption and desorption conditions for the resin, silicic acid, and evaluate the simultaneous fermentation and online recovery system for the production of human lysozyme by K. lactis K7 in biofilm reactor. The results demonstrated that the best adsorption (95.6% adsorption) was obtained at 25°C, pH 4, and 25% silicic acid ratio. Also, the best desorption parameters were determined as 25°C, pH -6.2, and 5% sodium dodecyl sulfate with 1 M NaCI and 20% ethanol as eluent composition. Four repetitive desorption process provided up to 98% desorption. Finally, the simultaneous fermentation and online recovery system with tangential flow microfilter module improved the production of human lysozyme to280.4 U/ml, which is 63% improvement compared to the biofilm bioreactor without online recovery system.

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M3 - Conference contribution

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Ercan D, Demirci A. Simultaneous online recovery of human lysozyme produced by kluyveromyces lactis K7 in biofilm reactor. In American Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014. American Society of Agricultural and Biological Engineers. 2014. p. 1318-1330. (American Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014).