TY - JOUR
T1 - Simultaneous quantification of jasmonic acid and salicylic acid in plants by vapor-phase extraction and gas chromatography-chemical ionization-mass spectrometry
AU - Engelberth, Juergen
AU - Schmelz, Eric A.
AU - Alborn, Hans T.
AU - Cardoza, Yasmin J.
AU - Huang, Juan
AU - Tumlinson, James H.
N1 - Funding Information:
We thank Dr. Hans Weber and Prof. Dr. Ralph O. Mumma for their critical reviews of the manuscript. This research was supported in part by a grant from the Defense Advanced Research Projects Agency (DARPA).
PY - 2003/1/15
Y1 - 2003/1/15
N2 - Jasmonic acid and salicylic acid represent important signaling compounds in plant defensive responses against other organisms. Here, we present a new method for the easy, sensitive, and reproducible quantification of both compounds by vapor-phase extraction and gas chromatography-positive ion chemical ionization-mass spectrometry. The method is based on a one-step extraction, phase partitioning, methylation with HCl/methanol, and collection of methylated and, thus, volatilized compounds on Super Q filters, thereby omitting further purification steps. Eluted samples are analyzed and quantified by GC/MS with chemical ionization. Standard curves were linear over a range of 5-1000 ng for jasmonic acid and salicylic acid. The correlation coefficients were greater than 0.999 and the recovery rates estimated between 70 and 90% for salicylic acid and 90 and 100% for jasmonic acid. The limit of detection was about 500 fg by using single ion detection mode. Both, cis- and trans-isomers for jasmonic acid can be detected. A comparison with established methods indicates the new method to be highly efficient, allowing reliable quantification of both compounds from small amounts of plant material (5-400 mg fresh weight).
AB - Jasmonic acid and salicylic acid represent important signaling compounds in plant defensive responses against other organisms. Here, we present a new method for the easy, sensitive, and reproducible quantification of both compounds by vapor-phase extraction and gas chromatography-positive ion chemical ionization-mass spectrometry. The method is based on a one-step extraction, phase partitioning, methylation with HCl/methanol, and collection of methylated and, thus, volatilized compounds on Super Q filters, thereby omitting further purification steps. Eluted samples are analyzed and quantified by GC/MS with chemical ionization. Standard curves were linear over a range of 5-1000 ng for jasmonic acid and salicylic acid. The correlation coefficients were greater than 0.999 and the recovery rates estimated between 70 and 90% for salicylic acid and 90 and 100% for jasmonic acid. The limit of detection was about 500 fg by using single ion detection mode. Both, cis- and trans-isomers for jasmonic acid can be detected. A comparison with established methods indicates the new method to be highly efficient, allowing reliable quantification of both compounds from small amounts of plant material (5-400 mg fresh weight).
UR - http://www.scopus.com/inward/record.url?scp=0037439517&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037439517&partnerID=8YFLogxK
U2 - 10.1016/S0003-2697(02)00466-9
DO - 10.1016/S0003-2697(02)00466-9
M3 - Article
C2 - 12531212
AN - SCOPUS:0037439517
VL - 312
SP - 242
EP - 250
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -