Single cell amperometry reveals glycocalyx hinders the release of neurotransmitters during exocytosis

Raphaël Trouillon, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

Abstract

The diffusional hindrance of the glycocalyx along the cell surface on exocytotic peaks, observed with single cell amperometry, was investigated. Partial digestion of the glycocalyx with neuraminidase led to the observation of faster peaks, as shown by varied peak parameters. This result indicates that diffusion of small molecules in the partially digested glycocalyx is 2.2 faster than in the intact glycocalyx. Similarly, neutralization of the negative charges present in the cell microenvironment led to faster peak kinetics. The analysis of the vesicular efflux indicates that the diffusion coefficient of dopamine at the cell surface is at most 45% of the diffusion coefficient in free solution. This study shows that the glycocalyx plays an important role in the diffusion kinetics of processes along the cell surface, including exocytotic events.

Original languageEnglish (US)
Pages (from-to)4822-4828
Number of pages7
JournalAnalytical Chemistry
Volume85
Issue number9
DOIs
StatePublished - May 7 2013

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Single cell amperometry reveals glycocalyx hinders the release of neurotransmitters during exocytosis'. Together they form a unique fingerprint.

Cite this