Single-Cell Analysis of Gene Expression by Fluorescence Microscopy

Research output: Chapter in Book/Report/Conference proceedingChapter

23 Citations (Scopus)

Abstract

Gene regulation by two-component systems has traditionally been studied using assays that involve averages over large numbers of cells. Single-cell measurements of transcription offer a complementary approach that provides the distribution of gene expression among the population. This chapter focuses on methods for using fluorescence microscopy and fluorescent proteins to study gene expression in single cells.

Original languageEnglish (US)
Title of host publicationTwo Component Signaling Systems, Part B
PublisherAcademic Press Inc.
Pages458-475
Number of pages18
DOIs
StatePublished - Jan 1 2007

Publication series

NameMethods in Enzymology
Volume423
ISSN (Print)0076-6879

Fingerprint

Single-Cell Analysis
Fluorescence microscopy
Fluorescence Microscopy
Gene expression
Gene Expression
Cell Count
Transcription
Assays
Population
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Miyashiro, T. I., & Goulian, M. (2007). Single-Cell Analysis of Gene Expression by Fluorescence Microscopy. In Two Component Signaling Systems, Part B (pp. 458-475). (Methods in Enzymology; Vol. 423). Academic Press Inc.. https://doi.org/10.1016/S0076-6879(07)23022-8
Miyashiro, Timothy Iwao ; Goulian, Mark. / Single-Cell Analysis of Gene Expression by Fluorescence Microscopy. Two Component Signaling Systems, Part B. Academic Press Inc., 2007. pp. 458-475 (Methods in Enzymology).
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Miyashiro, TI & Goulian, M 2007, Single-Cell Analysis of Gene Expression by Fluorescence Microscopy. in Two Component Signaling Systems, Part B. Methods in Enzymology, vol. 423, Academic Press Inc., pp. 458-475. https://doi.org/10.1016/S0076-6879(07)23022-8

Single-Cell Analysis of Gene Expression by Fluorescence Microscopy. / Miyashiro, Timothy Iwao; Goulian, Mark.

Two Component Signaling Systems, Part B. Academic Press Inc., 2007. p. 458-475 (Methods in Enzymology; Vol. 423).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Miyashiro TI, Goulian M. Single-Cell Analysis of Gene Expression by Fluorescence Microscopy. In Two Component Signaling Systems, Part B. Academic Press Inc. 2007. p. 458-475. (Methods in Enzymology). https://doi.org/10.1016/S0076-6879(07)23022-8