Single cell antimicrobial susceptibility testing using confined microchannels and electrokinetic loading

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This study reports a single cell antimicrobial susceptibility testing (AST) technique using confined microchannels and electrokinetic loading. By confining individual bacteria in gas permeable microchannels with dimensions comparable to a single bacteria (~2 μm), their growth can be monitored with high resolution at the single cell level. Furthermore, AC electrokinetics is successfully demonstrated in high-conductivity culture media to facilitate dynamic loading of bacterial pathogens into the confined microchannels with over 80% loading efficiency. The single cell AST technique allows antibiotic resistance profiling to be finished in less than one hour compared to days in standard culture based techniques.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1050-1052
Number of pages3
StatePublished - Dec 1 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Fingerprint

Microchannels
Bacteria
Testing
Pathogens
Antibiotics
Gases

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Lu, Y., & Wong, P. K. (2011). Single cell antimicrobial susceptibility testing using confined microchannels and electrokinetic loading. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1050-1052). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).
Lu, Yi ; Wong, Pak Kin. / Single cell antimicrobial susceptibility testing using confined microchannels and electrokinetic loading. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. pp. 1050-1052 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).
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abstract = "This study reports a single cell antimicrobial susceptibility testing (AST) technique using confined microchannels and electrokinetic loading. By confining individual bacteria in gas permeable microchannels with dimensions comparable to a single bacteria (~2 μm), their growth can be monitored with high resolution at the single cell level. Furthermore, AC electrokinetics is successfully demonstrated in high-conductivity culture media to facilitate dynamic loading of bacterial pathogens into the confined microchannels with over 80{\%} loading efficiency. The single cell AST technique allows antibiotic resistance profiling to be finished in less than one hour compared to days in standard culture based techniques.",
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Lu, Y & Wong, PK 2011, Single cell antimicrobial susceptibility testing using confined microchannels and electrokinetic loading. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, vol. 2, pp. 1050-1052, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 10/2/11.

Single cell antimicrobial susceptibility testing using confined microchannels and electrokinetic loading. / Lu, Yi; Wong, Pak Kin.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1050-1052 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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N2 - This study reports a single cell antimicrobial susceptibility testing (AST) technique using confined microchannels and electrokinetic loading. By confining individual bacteria in gas permeable microchannels with dimensions comparable to a single bacteria (~2 μm), their growth can be monitored with high resolution at the single cell level. Furthermore, AC electrokinetics is successfully demonstrated in high-conductivity culture media to facilitate dynamic loading of bacterial pathogens into the confined microchannels with over 80% loading efficiency. The single cell AST technique allows antibiotic resistance profiling to be finished in less than one hour compared to days in standard culture based techniques.

AB - This study reports a single cell antimicrobial susceptibility testing (AST) technique using confined microchannels and electrokinetic loading. By confining individual bacteria in gas permeable microchannels with dimensions comparable to a single bacteria (~2 μm), their growth can be monitored with high resolution at the single cell level. Furthermore, AC electrokinetics is successfully demonstrated in high-conductivity culture media to facilitate dynamic loading of bacterial pathogens into the confined microchannels with over 80% loading efficiency. The single cell AST technique allows antibiotic resistance profiling to be finished in less than one hour compared to days in standard culture based techniques.

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Lu Y, Wong PK. Single cell antimicrobial susceptibility testing using confined microchannels and electrokinetic loading. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1050-1052. (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).